Expression of two green fluorescent protein variants in citrate-buffered media in Pichia pastoris

被引：6

作者：

Campbell, TN ^{[1
]}

Choy, FYM ^{[1
]}

机构：

[1] Univ Victoria, Biomed Res Ctr, Dept Biol, Victoria, BC V8W 3N5, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/S0003-2697(02)00409-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：193 / 195

页数：3

共 9 条

[1]

Campbell Tessa N., 2001, Molecular Biology Today, V2, P1

[2]

CHALFIE M, 1998, GREEN FLUORESCENT PR

[3] Nuclear export of 60S ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p that associates with the large subunit protein Rpl10p [J].

Gadal, O ;

Strauss, D ;

Kessl, J ;

Trumpower, B ;

Tollervey, D ;

Hurt, E .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (10) :3405-3415

[4]

GLEW RH, 1977, LAB RES METHODS BIOL, V1, P84

[5] CODON FREQUENCIES IN 119 INDIVIDUAL GENES CONFIRM CONSISTENT CHOICES OF DEGENERATE BASES ACCORDING TO GENOME TYPE [J].

GRANTHAM, R ;

GAUTIER, C ;

GOUY, M .

NUCLEIC ACIDS RESEARCH, 1980, 8 (09) :1893-1912

[6] Codon usage tabulated from the international DNA sequence databases; its status 1999 [J].

Nakamura, Y ;

Gojobori, T ;

Ikemura, T .

NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :292-292

[7] Optimizing functional versus total expression of the human μ-opioid receptor in Pichia pastoris [J].

Sarramegna, V ;

Demange, P ;

Milon, A ;

Talmont, F .

PROTEIN EXPRESSION AND PURIFICATION, 2002, 24 (02) :212-220

[8] Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses [J].

Shibasaki, S ;

Ueda, M ;

Iizuka, T ;

Hirayama, M ;

Ikeda, Y ;

Kamasawa, N ;

Osumi, M ;

Tanaka, A .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 55 (04) :471-475

[9]

Spector D.L., 1998, Cells : a laboratory manual

← 1 →