Trigonelline attenuates hepatic complications and molecular alterations in high-fat high-fructose diet-induced insulin resistance in rats

被引:23
作者
Afifi, Nehal A. [1 ]
Ramadan, Amer [1 ]
Erian, Emad Y. [2 ]
Saleh, Dalia O. [2 ]
Sedik, Ahmed A. [2 ]
Badawi, Manal [3 ]
El Hotaby, Walid [4 ]
机构
[1] Cairo Univ, Fac Vet Med, Pharmacol Dept, Giza, Egypt
[2] Natl Res Ctr, Pharmacol Dept, Cairo, Egypt
[3] Natl Res Ctr, Pathol Dept, Cairo, Egypt
[4] Natl Res Ctr, Biophys Dept, Cairo, Egypt
关键词
trigonelline; sitagliptin; insulin resistance; liver; molecular alterations; TUMOR-NECROSIS-FACTOR; INDUCED LIVER-INJURY; NITRIC-OXIDE; METABOLIC ALTERATIONS; LIPID-PEROXIDATION; DIABETES-MELLITUS; WISTAR RATS; DISEASE; GLUCOSE; MECHANISMS;
D O I
10.1139/cjpp-2016-0269
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The present study aimed to evaluate the effect of trigonelline (TRG) on the hepatic complications associated with high-fat high-fructose (HFHF) diet-induced insulin resistance (IR) in rats. IR was induced by giving a saturated fat diet and 10% fructose in drinking water to rats for 8 weeks. Insulin-resistant rats were orally treated with TRG (50 and 100 mg/kg), sitagliptin (SIT; 5 mg/kg), or a combination of TRG (50 mg/kg) and SIT (5 mg/kg) for 14 days. Liver homogenates were used for assessment of hepatic lipids, oxidative stress biomarkers, and inflammatory cytokines. Histopathological and DNA cytometry examinations were carried out for hepatic and pancreatic tissues. Hepatic tissues were examined using Fourier-transform infrared spectroscopy for assessment of any molecular changes. Results of the present study revealed that oral treatment of insulin-resistant rats with TRG or TRG in combination with SIT significantly decreased homeostatic model assessment of IR, hepatic lipids, oxidative stress biomarkers, and the inflammatory cytokines. TRG or TRG in combination with SIT ameliorated the histopathological, DNA cytometry, and molecular alterations induced by a HFHF diet. Finally, it can be concluded that TRG has beneficial effects on the hepatic complications associated with IR due to its hypoglycemic effect and antioxidant potential.
引用
收藏
页码:427 / 436
页数:10
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