Effect of Preparation of β2M Nano Antibody Adsorbent for Blood Purification on Dialysis Complications in Patients with Renal Failure

被引：0

作者：

Peng, Jianyun ^{[1
]}

Tao, Yongling ^{[2
]}

Zhang, Xiaoru ^{[1
]}

Xiang, Meijuan ^{[1
]}

Gui, Zhihong ^{[1
]}

Wu, Jing ^{[3
]}

Yin, Liqing ^{[4
]}

机构：

[1] Wenzhou Med Univ, Lishui Peoples Hosp, Dept Nephrol, Affiliated Hosp 6, Lishui 323000, Peoples R China

[2] Wenzhou Med Univ, Lishui Peoples Hosp, Dept Pathol, Affiliated Hosp 6, Lishui 323000, Peoples R China

[3] Wenzhou Med Univ, Lishui Peoples Hosp, Dept Blood Purificat, Affiliated Hosp 6, Lishui 323000, Peoples R China

[4] Yantai Haigang Hosp, Dept Nephrol, Yantai 264000, Shandong, Peoples R China

来源：

SCIENCE OF ADVANCED MATERIALS | 2021年 / 13卷 / 01期

关键词：

Renal Failure; beta M-2; A53S; Nanoantibody Screening; Dynamic Adsorption Capacity; BETA-2-MICROGLOBULIN; BETA(2)-MICROGLOBULIN; MORTALITY; SERUM; RISK; IGG;

D O I：

10.1166/sam.2021.3881

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In order to purify blood and adsorb beta M-2, a highly selective immunosorbent biomaterial based on nano antibody was designed. First of all, nanoantibodies against beta M-2 in patients with renal failure are screened by phage display library technology. After 4 rounds of screening, 12 kinds of monoclonal phage samples are compared by ELISA, and the highest affinity sequence A53 is obtained. Then, the nano antibody A53 is transferred into two kinds of host bacteria respectively, and the host E. coli Shuffle T7 is selected, and then purified by nickel column affinity, thus obtaining high purity nano antibody A53S. Five kinds of nano antibody adsorbents are prepared by using A53S as a component of adsorbent. The results show that the effect of D series adsorbent with epsilon-poly-L-lysine as spacer is better, but the ligand density should be adjusted reasonably to ensure the activity of nanoantibody protein. When the loading concentration of beta M-2 is set to 1.8 mg/ml, adsorbent D-1 can adsorb 11.78 mg/ml of dynamic adsorption capacity of beta M-2. This index is significantly higher than the dynamic adsorption capacity of plasma system under the same conditions (P < 0.05), which proves that the nano antibody adsorbent biomaterial can effectively adsorb beta M-2, thus reducing the probability of dialysis complications.

引用

页码：161 / 170

页数：10