The investigation of the efficacy ratio of cress seeds supplementation to moderate hyperglycemia and hepatotoxicity in streptozotocin-induced diabetic rats

被引:6
作者
Doghmane, Amina [1 ]
Aouacheri, Ouassila [1 ,2 ]
Laouaichia, Rania [1 ]
Saka, Saad [1 ,2 ]
机构
[1] Univ Badji Mokhtar, Dept Biochem, Lab Appl Biochem & Microbiol, Annaba, Algeria
[2] Univ Badji Mokhtar, Dept Biol, Lab Anim Ecophysiol, Annaba, Algeria
关键词
Lepidium sativum; Diabetes; Hepatoprotective; Oxidative stress; Rats; OXIDATIVE STRESS; ANTIDIABETIC ACTIVITY; FERULIC ACID; LIVER; EXTRACT; MELLITUS; MECHANISMS; TOXICITY; TISSUES; IMPACT;
D O I
10.1007/s40200-021-00764-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective Oxidative stress resulting from chronic hyperglycemia induced many complications in diabetes and led to disorders and dysfunctions in different organs. This study aimed to evaluate the hepatoprotective rate of cress seeds (CS) or Lepidium sativum seeds in the diet on lowering hyperglycemia and oxidative stress damaging. Methods Diabetes was induced by a single intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). Forty-eight male rats were randomly divided into six groups : (D-0) and (ND-0) diabetic, and non-diabetic groups were fed with a normal diet, (ND-CS2) and (ND-CS5) non-diabetic groups were fed with diet containing 2 % and 5 % of cress seeds respectively, (D-CS2) and (D-CS5) diabetic groups were fed with diet containing 2 % and 5 % of cress seeds respectively. After 28 days of treatment, biochemical, histological, and oxidative parameters were determined. Hepatic and pancreatic histological sections were developed. Results STZ-injection caused hyperglycemia accompanied by a disturbance in biochemical parameters and intensified oxidative stress status compared to the (ND-0) group. Hepatic and pancreatic histological sections of diabetic rats showed a disrupted architecture. However, the cress seeds-diet revealed a significant decrease of hyperglycemia and a reduction of the intensity of oxidative stress induced by diabetes compared to the (D-0) group, remarked by a decreased level of Malondialdehyde (MDA) and high levels of glutathione (GSH) and the antioxidant enzymes, led to the decrease of the majority of parameters principally hepatic and lipid profile with histological regeneration. Conclusions Cress seeds supplementation confirmed their potential anti-diabetic and antioxidant activities with higher efficacy of 5 % dose than the lower dose of 2 %. Therefore, 5 % of cress seeds administration seems to be the excellent rate recommended in controlling diabetes and its complications.
引用
收藏
页码:447 / 459
页数:13
相关论文
共 69 条
[1]   Modulation of the lipid profile and insulin levels of streptozotocin induced diabetic rats by ethanol extract of Cnidoscolus aconitifolius leaves and some fractions: Effect on the oral glucose tolerance of normoglycemic rats [J].
Achi, N. K. ;
Ohaeri, O. C. ;
Ijeh, I. I. ;
Eleazu, C. .
BIOMEDICINE & PHARMACOTHERAPY, 2017, 86 :562-569
[2]   Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage [J].
Adeyemi, David O. ;
Ukwenya, Victor O. ;
Obuotor, Efere M. ;
Adewole, Stephen O. .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2014, 14
[3]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[4]   Flavonoids and Their Anti-Diabetic Effects: Cellular Mechanisms and Effects to Improve Blood Sugar Levels [J].
AL-Ishaq, Raghad Khalid ;
Abotaleb, Mariam ;
Kubatka, Peter ;
Kajo, Karol ;
Busselberg, Dietrich .
BIOMOLECULES, 2019, 9 (09)
[5]  
Al-khazraji SM., 2012, IRAQI J VET MED, V36, P158
[6]   Protective effect of Lepidium sativum seed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2) [J].
Al-Sheddi, Ebtesam S. ;
Farshori, Nida N. ;
Al-Oqail, Mai M. ;
Musarrat, Javed ;
Al-Khedhairy, Abdulaziz A. ;
Siddiqui, Maqsood A. .
PHARMACEUTICAL BIOLOGY, 2016, 54 (02) :314-321
[7]  
Alharbi F. K., 2017, J CHEM ENV HLTH, V3, P1
[8]  
Amawi K., 2012, Journal of Physiology and Pharmacology Advances, V2, P277
[9]  
[Anonymous], 2014, ANTIOXIDANT ANTIDIAB, DOI [10.5772/57282, DOI 10.5772/57282]
[10]   The Investigation of the Oxidative Stress-Related Parameters in Type 2 Diabetes Mellitus [J].
Aouacheri, Ouassila ;
Saka, Saad ;
Krim, Meriem ;
Messaadia, Amira ;
Maidi, Imen .
CANADIAN JOURNAL OF DIABETES, 2015, 39 (01) :44-49