Multisite recording of extracellular potentials produced by microchannel-confined neurons in-vitro

Towards establishing electrical interfaces with patterned in vitro neurons, we have previously described the fabrication of hybrid elastomer-glass devices polymer-on-multielectrode array technology and obtained single-electrode recordings of extracellular potentials from confined neurons (Claverol-Tinture et al, 2005). Here, we demonstrate the feasibility of spatially localized multisite recordings from individual microcharmel-guided neurites extending from microwell-confined somas with good signal-to-noise ratios (20 dB) and spike magnitudes of up to 300 mu V. Single-cell current source density (scCSD) analysis of the spatio-temporal patterns of membrane currents along individual processes is illustrated.