Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure

被引:52
作者
Bharati, Jaya [1 ]
Dangi, S. S. [1 ]
Mishra, S. R. [1 ]
Chouhan, V. S. [1 ]
Verma, V. [1 ]
Shankar, O. [1 ]
Bharti, M. K. [1 ]
Paul, A. [1 ]
Mahato, Dilip K. [1 ]
Rajesh, G. [1 ]
Singh, G. [1 ]
Maurya, V. P. [1 ]
Bag, S. [1 ]
Kumar, Puneet [1 ]
Sarkar, M. [1 ]
机构
[1] Indian Vet Res Inst, Physiol & Climatol Div, Bareilly 243122, Uttar Pradesh, India
关键词
Heat stress; Interleukin; PBMCs; Toll like receptor; Tharparkar cattle; BUFFALOS BUBALUS-BUBALIS; SHOCK RESPONSE; CAPRA-HIRCUS; BOS-INDICUS; CORTISOL; GENES; GOATS; CELLS; TEMPERATURES; ACTIVATION;
D O I
10.1016/j.jtherbio.2017.02.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Six male Tharparkar cattle of 2-3 years old were selected for the study. After 15 days acclimation at thermo neutral zone (TNZ) in psychrometric chamber, animals were exposed at 42 degrees C for 6 h up to 23 days followed by 12 days of recovery period. Blood samples were collected during control period at TNZ (day 1, 5 and 12), after heat stress exposure (day 1-10, Short Term Heat Stress Acclimation-STHSA; day 15-23, Long Term Heat Stress Acclimation-LTHSA) and recovery period (day 7 and 12) and peripheral blood mononuclear cells (PBMCs) were isolated for RNA and protein extraction. Serum cortisol concentration was assessed by RIA. The mRNA and protein expression in PBMCs were determined by qPCR and western blot respectively. Samples at TNZ were taken as control. Serum cortisol concentration was increased (P < 0.05) during STHSA and gradually declined during LTHSA. Toll like receptor 2 (TLR 2) expression was up regulated (P < 0.05) during STHSA and declined to basal level during LTHSA and recovery phase. However, toll like receptor 4 (TLR 4) expression was up regulated (P < 0.05) during STHSA and LTHSA while declined in recovery phase. Interleukin 2 (IL2) and interleukin 6 (IL 6) were up regulated (P < 0.05) during STHSA and reduced to basal level during LTHSA. PBMCs culture study was conducted to study transcriptional abundance of TLR2/4 and IL2/6 at different temperature-time combinations. The present findings indicate that TLR 2/4 and IL 2/6 could possibly play a vital role in thermo tolerance in Tharparkar cattle during short term and long term heat stress exposure.
引用
收藏
页码:48 / 56
页数:9
相关论文
共 55 条
[1]  
ABILAY TA, 1975, J ANIM SCI, V41, P113
[2]   Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity [J].
Beatty, DT ;
Barnes, A ;
Taylor, E ;
Pethick, D ;
McCarthy, M ;
Maloney, SK .
JOURNAL OF ANIMAL SCIENCE, 2006, 84 (04) :972-985
[3]   UPPER CRITICAL-TEMPERATURES AND FORCED VENTILATION EFFECTS FOR HIGH-YIELDING DAIRY-COWS IN A SUB-TROPICAL CLIMATE [J].
BERMAN, A ;
FOLMAN, Y ;
KAIM, M ;
MAMEN, M ;
HERZ, Z ;
WOLFENSON, D ;
ARIELI, A ;
GRABER, Y .
JOURNAL OF DAIRY SCIENCE, 1985, 68 (06) :1488-1495
[4]  
Bharati J., 2016, INT J BIOMETEOROL
[6]  
BIANCA W., 1959, JOUR AGRIC SCI, V52, P305, DOI 10.1017/S0021859600029609
[7]  
Bligh J., 1973, TEMPERATURE REGULATI, P359
[8]   Heat shock up-regulates TLR9 expression in human B cells through activation of ERK and NF-κB signal pathways [J].
Chen, WL ;
Wang, JL ;
An, HZ ;
Zhou, J ;
Zhang, LH ;
Cao, XT .
IMMUNOLOGY LETTERS, 2005, 98 (01) :153-159
[9]  
CHRISTISON GI, 1972, J ANIM SCI, V35, P1005
[10]   Invited review: Genes involved in the bovine heat stress response [J].
Collier, R. J. ;
Collier, J. L. ;
Rhoads, R. P. ;
Baumgard, L. H. .
JOURNAL OF DAIRY SCIENCE, 2008, 91 (02) :445-454