Structural organization of the RNA polymerase-promoter open complex

被引：159

作者：

Naryshkin, N ^{[1
]}

Revyakin, A ^{[1
]}

Kim, YG ^{[1
]}

Mekler, V ^{[1
]}

Ebright, RH ^{[1
]}

机构：

[1] Rutgers State Univ, Waksman Inst, Howard Hughes Med Inst, Dept Chem, Piscataway, NJ 08854 USA

来源：

CELL | 2000年 / 101卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(00)80872-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.

引用

页码：601 / 611

页数：11

共 58 条

[1]

ALLEN G, 1989, SEQUENCING PROTEINS

[2]

ARTHUR T, 2000, IN PRESS J BIOL CHEM

[3] Localization of a σ70 binding site on the N terminus of the Escherichia coli RNA polymerase β′ subunit [J].

Arthur, TM ;

Burgess, RR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (47) :31381-31387

[4] DOMAIN ORGANIZATION OF RNA-POLYMERASE ALPHA-SUBUNIT - C-TERMINAL-85 AMINO-ACIDS CONSTITUTE A DOMAIN CAPABLE OF DIMERIZATION AND DNA-BINDING [J].

BLATTER, EE ;

ROSS, W ;

TANG, H ;

GOURSE, RL ;

EBRIGHT, RH .

CELL, 1994, 78 (05) :889-896

[5] Organization of open complexes at Escherichia coli promoters -: Location of promoter DNA sites close to region 2.5 of the σ70 subunit of RNA polymerase [J].

Bown, JA ;

Owens, JT ;

Meares, CF ;

Fujita, N ;

Ishihama, A ;

Busby, SJW ;

Minchin, SD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2263-2270

[6] Identification of RNA polymerase β′ subunit segment contacting the melted region of the lacUV5 promoter [J].

Brodolin, K ;

Mustaev, A ;

Severinov, K ;

Nikiforov, V .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (05) :3661-3666

[7] The kinetics of sigma subunit directed promoter recognition by E-coli RNA polymerase [J].

Buckle, M ;

Pemberton, IK ;

Jacquet, MA ;

Buc, H .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (03) :955-964

[8] DNA sequence elements located immediately upstream of the -10 hexamer in Escherichia coli promoters:: a systematic study [J].

Burr, T ;

Mitchell, J ;

Kolb, A ;

Minchin, S ;

Busby, S .

NUCLEIC ACIDS RESEARCH, 2000, 28 (09) :1864-1870

[9] Transcription activation by catabolite activator protein (CAP) [J].

Busby, S ;

Ebright, RH .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 293 (02) :199-213

[10] PROMOTER STRUCTURE, PROMOTER RECOGNITION, AND TRANSCRIPTION ACTIVATION IN PROKARYOTES [J].

BUSBY, S ;

EBRIGHT, RH .

CELL, 1994, 79 (05) :743-746

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