Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I

被引：11

作者：

Stettler, Alexandra R. ^{[1
]}

Chaurin, Valerie ^{[1
]}

Constable, Edwin C. ^{[1
]}

Housecroft, Catherine E. ^{[1
]}

Schwarz, Maria A. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

来源：

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | 2007年 / 12卷 / 02期

关键词：

affinity capillary electrophoresis; buffer; oligonucleotide; metal cation;

D O I：

10.1007/s00775-006-0180-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.

引用

页码：194 / 203

页数：10

共 58 条

[1] Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2 [J].

Abrescia, NGA ;

Malinina, L ;

Subirana, JA .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (03) :657-666

[2] Structure of the oligonucleotide d(CGTATATACG) as a site-specific complex with nickel ions [J].

Abrescia, NGA ;

Malinina, L ;

Fernandez, LG ;

Huynh-Dinh, T ;

Neidle, S ;

Subirana, JA .

NUCLEIC ACIDS RESEARCH, 1999, 27 (07) :1593-1599

[3] Nickel-guanine interactions in DNA:: crystal structure of nickel-d[CGTGTACACG]2 [J].

Abrescia, NGA ;

Huynh-Dinh, T ;

Subirana, JA .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2002, 7 (1-2) :195-199

[4] A comparative study of DNA complexation with Mg(II) and Ca(II) in aqueous solution: Major and minor grooves bindings [J].

Ahmad, R ;

Arakawa, H ;

Tajmir-Riahi, HA .

BIOPHYSICAL JOURNAL, 2003, 84 (04) :2460-2466

[5] Ternary complexes in solution. Comparison of the coordination tendency of some biologically important zwitterionic buffers toward the binary complexes of some transition metal ions and some amino acids [J].

Anwar, ZM ;

Azab, HA .

JOURNAL OF CHEMICAL AND ENGINEERING DATA, 1999, 44 (06) :1151-1157

[6] Silver(I) complexes with DNA and RNA studied by Fourier transform infrared spectroscopy and capillary electrophoresis [J].

Arakawa, H ;

Neault, JF ;

Tajmir-Riahi, HA .

BIOPHYSICAL JOURNAL, 2001, 81 (03) :1580-1587

[7] Role of biologically important zwitterionic buffer secondary ligands on the stability of the mixed-ligand complexes of divalent metal ions and adenosine 5′-mono-, 5′-di-, and 5′-triphosphate [J].

Azab, HA ;

Orabi, AS ;

El-Salam, ETA .

JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2001, 46 (02) :346-354

[8] INTERACTION OF 2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL WITH COPPER(2) AND NICKEL(2) IONS [J].

BAI, KS ;

MARTELL, AE .

JOURNAL OF INORGANIC & NUCLEAR CHEMISTRY, 1969, 31 (06) :1697-&

[9] Divalent transition metal cations counteract potassium-induced quadruplex assembly of oligo(dG) sequences [J].

Blume, SW ;

Guarcello, V ;

Zacharias, W ;

Miller, DM .

NUCLEIC ACIDS RESEARCH, 1997, 25 (03) :617-625

[10] COMPLEX-FORMATION EQUILIBRIA BETWEEN 2-AMINO-2(HYDROXYMETHYL)-1,3-PROPANEDIOL (TRIS, THAM) AND NICKEL(II), COPPER(II), ZINC(II) AND HYDROGEN-IONS IN AQUEOUS-SOLUTIONS [J].

BOLOGNI, L ;

SABATINI, A ;

VACCA, A .

INORGANICA CHIMICA ACTA-ARTICLES, 1983, 69 (FEB) :71-75

← 1 2 3 4 5 6 →