Scanning near-field optical microscope: A method for investigating chromosomes

被引：0

作者：

Wiegrabe, W ^{[1
]}

Monajembashi, S ^{[1
]}

Dittmar, H ^{[1
]}

Greulich, KO ^{[1
]}

Hafner, S ^{[1
]}

Hildebrandt, M ^{[1
]}

Kittler, M ^{[1
]}

Lochner, B ^{[1
]}

Unger, E ^{[1
]}

机构：

[1] INST MOL BIOTECHNOL EV,D-07745 JENA,GERMANY

来源：

SURFACE AND INTERFACE ANALYSIS | 1997年 / 25卷 / 7-8期

关键词：

chromosome; DNA; karyotype; Giemsa; propidium iodide; scanning near-held optical microscope; SNOM; NSOM; scanning force microscope; SFM; AFM; ATOMIC FORCE MICROSCOPE; INSITU HYBRIDIZATION;

D O I：

10.1002/(SICI)1096-9918(199706)25:7/8<510::AID-SIA260>3.0.CO;2-S

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Karyotypes of human metaphase chromosomes are used to detect genetic defects like deletions or translocations. For these investigations the chromosomes are treated by the trypsin-Giemsa protocol, resulting in a typical banding pattern. These patterns are investigated using conventional light microscopy. Because of the diffraction limit, even the smallest visible band contains similar to 1 million base pairs. We want to improve resolution by using bright-field scanning near-field optical microscopy (SNOM). Images of trypsin-Giemsa-treated chromosomes are presented and compared with conventional light microscopic, scanning force and scanning fluorescence near-field optical microscopic data. For fluorescence investigations, the chromosomes were stained using propidium iodide. To our knowledge, it is the first attempt to investigate G-banded chromosomes by SNOM. (C) 1997 by John Wiley & Sons, Ltd.

引用

页码：510 / &

页数：5

共 19 条

[1]

Ambrose W. P., 1995, Experimental Technique of Physics, V41, P237

[2] NEAR-FIELD OPTICS - MICROSCOPY, SPECTROSCOPY, AND SURFACE MODIFICATION BEYOND THE DIFFRACTION LIMIT [J].