Interactions between HIV-1 protease, silver nanoparticles, and specific peptides

被引：14

作者：

Tsai, Chieh-Hsuan ^{[1
]}

Whiteley, Chris G. ^{[2
]}

Lee, Duu-Jong ^{[1
,3
,4
,5
]}

机构：

[1] Natl Taiwan Univ, Dept Chem Engn, Taipei 10617, Taiwan

[2] Rhodes Univ, Dept Biochem & Microbiol, Grahamstown, South Africa

[3] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 10607, Taiwan

[4] Tunghai Univ, Coll Engn, Taichung 40704, Taiwan

[5] Natl Taiwan Normal Univ, Coll Technol & Engn, Taipei 10610, Taiwan

来源：

JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS | 2019年 / 103卷

关键词：

Silver nanoparticles; HIV-1; protease; Inhibition; Cleavage; HUMAN-IMMUNODEFICIENCY-VIRUS; ACTIVE-SITE; SUBSITE PREFERENCES; PROTEINASE; INHIBITORS; SUBSTRATE;

D O I：

10.1016/j.jtice.2019.07.019

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Use of silver nanoparticles (Ag NP's) as inhibitor for HIV-1 protease (PR) has practical interest. However, the interactions between the Ag NP's, HIV-1 PR, and the target peptides are not well demonstrated. This study experimentally revealed the interactions of the synthesized Ag NP's, HIV-1 PR, and five specific peptides with similar amino acid sequences to HIV's polyproteins. The Ag NP's can bind with either HIV-1 PR or the peptides. Incubating Ag NP's with peptide for 24 h can efficiently inhibit activity of HIV-1 PR. But HIV-1 PR would cleave peptides faster than the binding rate of Ag NP's and the peptides; therefore, late presence of Ag NP's would have no inhibition on the HIV-1 PR activity. (C) 2019 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

引用

页码：20 / 32

页数：13

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