Discrete determinants in transfer RNA for editing and aminoacylation

被引：84

作者：

Hale, SP ^{[1
]}

Auld, DS ^{[1
]}

Schmidt, E ^{[1
]}

Schimmel, P ^{[1
]}

机构：

[1] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

来源：

SCIENCE | 1997年 / 276卷 / 5316期

关键词：

D O I：

10.1126/science.276.5316.1250

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During translation errors of aminoacylation are corrected in editing reactions which ensure that an amino acid is stably attached to its corresponding transfer RNA (tRNA). Previous studies have not shown whether the tRNA nucleotides needed for effecting translational editing are the same as or distinct from those required for aminoacylation, but several considerations have suggested that they are the same. Here, designed tRNAs that are highly active for aminoacylation but are not active in translational editing are presented. The editing reaction can be controlled by manipulation of nucleotides at the corner of the L-shaped tRNA. In contrast, these manipulations do not affect aminoacylation. These results demonstrate the segregation of nucleotide determinants for the editing and aminoacylation functions of tRNA.

引用

页码：1250 / 1252

页数：3

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