Electrochemical investigations of corticosteroid isomers-testosterone and epitestosterone and their simultaneous determination in human urine

被引：43

作者：

Goyal, Rajendra N. ^{[1
]}

Gupta, Vinod K. ^{[1
,2
]}

Chatterjee, Sanghamitra ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh, India

[2] King Fahd Univ Petr & Minerals, Dhahran 31261, Saudi Arabia

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 657卷 / 02期

关键词：

Doping; Single-wall carbon nanotubes; Edge plane pyrolytic graphite electrode; Testosterone; Epitestosterone; Human urine; CHROMATOGRAPHY-MASS SPECTROMETRY; LIQUID-CHROMATOGRAPHY; VOLTAMMETRIC DETERMINATION; BIOLOGICAL-FLUIDS; ANABOLIC-STEROIDS; QUANTIFICATION; ELECTRODE; SAMPLES; RATIO; ADENOSINE;

D O I：

10.1016/j.aca.2009.10.035

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Voltammetric investigation of two corticoid isomers-testosterone and epitestosterone has been carried out at bare and single-wall carbon nanotubes (SWNT)-modified edge plane pyrolytic graphite electrode (EPPGE). Square wave voltammetry (OSWV) has been used for the simultaneous determination of isomeric steroids. The reduction of the two isomers occurred in a pH dependent, 2e, 2H(+) process and well-defined voltammetric peaks were observed. Under the optimum experimental conditions, linear calibration curves are obtained within the concentration range 5-1000 nM for both the steroids with the limit of detection 2.8 x 10(-9) and 4.1 x 10(-9) M for testosterone and epitestosterone respectively. The developed protocol is successfully implemented for the analysis of both the compounds in the urine samples of normal subjects as well as in patients undergoing treatment with testosterone. The results obtained from the proposed voltammetric method were also compared with HPLC analysis and found to be similar. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：147 / 153

页数：7

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