Novel optical pH-sensor based on a boradiaza-indacene derivative

被引：94

作者：

Werner, T ^{[1
]}

Huber, C ^{[1
]}

Heinl, S ^{[1
]}

Kollmannsberger, M ^{[1
]}

Daub, J ^{[1
]}

Wolfbeis, OS ^{[1
]}

机构：

[1] UNIV REGENSBURG,INST ORGAN CHEM,D-93040 REGENSBURG,GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1997年 / 359卷 / 02期

关键词：

Signal Intensity; Electron Transfer; High Signal; High Signal Intensity; Good Reproducibility;

D O I：

10.1007/s002160050552

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A pH-optode is described based on 1,3,5,7-tetramethyl-8-(4-dimethylamino)-4-difluorobora-3a,4a-diaza-(s)-indacene (1) immobilized in a hydrogel matrix. Its uncharged form is non-fluorescent, whereas the protonated form displays strong emission. The quenching of the emission can be mechanistically explained by a PET (photoinduced electron transfer) from the dimethylamino group to the fluorophore. The perpendicular configuration at the C(8)-aryl bond contributes to the efficiency of the on/off-switching behavior. Incorporation of the dye into a hydrogel matrix results in a transparent sensor membrane which can be used to measure the pH in the range of 1-3. The sensor shows high signal intensity, good reproducibility, and the response characteristic is stable over hours of operation even at low pH-values.

引用

页码：150 / 154

页数：5

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