CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis

被引：161

作者：

Ai, Jing-Wen ^{[1
]}

Zhou, Xian ^{[1
]}

Xu, Teng ^{[2
]}

Yang, Minling ^{[2
]}

Chen, Yuanyuan ^{[3
]}

He, Gui-Qing ^{[4
]}

Pan, Ningp ^{[4
]}

Cai, Yuwei ^{[4
]}

Li, Yongjun ^{[2
]}

Wang, Xiaorui ^{[2
]}

Su, Hang ^{[2
]}

Wang, Ting ^{[5
]}

Zeng, Weiqi ^{[2
]}

Zhang, Wen-Hong ^{[1
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Infect Dis, 12 Wulumuqi Zhong Rd, Shanghai 200040, Peoples R China

[2] Vis Med Co Ltd, G10 BLDG,Huaxin Pk,31 Kefeng Ave, Guangzhou 510000, Guangdong, Peoples R China

[3] Hangzhou Red Cross Hosp, TB Diag & Treatment Ctr, Hangzhou, Zhejiang, Peoples R China

[4] Wenzhou Cent Hosp, Dept Infect Dis, Wenzhou, Peoples R China

[5] Jingan Dist Cent Hosp Shanghai, Dept Infect Dis, Shanghai, Peoples R China

来源：

EMERGING MICROBES & INFECTIONS | 2019年 / 8卷 / 01期

关键词：

CRISPR-MTB; tuberculosis; Mycobacterium tuberculosis complex (MTB); GeneXpert MTB; RIF; diagnosis; ADAPTIVE IMMUNITY; DNA;

D O I：

10.1080/22221751.2019.1664939

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Rapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent development has unveiled the diagnostic power of the CRISPR system in the detection of viral infections. However, its potential use in detecting the Mycobacterium tuberculosis complex (MTB) remained unexplored. We developed a rapid CRISPR-based assay for TB detection and conducted a retrospective cohort study of 179 patients to evaluate the CRISPR-MTB test for identifying MTB in various forms of direct clinical samples. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the clinical cohort of both pulmonary and extra-pulmonary tuberculosis, the CRISPR-MTB test exhibited an overall improved sensitivity over both culture (79% vs 33%) and Xpert (79% vs 66%), without comprise in specificity (62/63, 98%). The CRISPR-MTB test exhibits an improved overall diagnostic performance over culture and Xpert across a variety of sample types, and offers great potential as a new diagnostic technique for both pulmonary and extra-pulmonary tuberculosis.

引用

页码：1361 / 1369

页数：9

共 21 条

[11] CRISPR-Cas: Evolution of an RNA-based adaptive immunity system in prokaryotes [J].

Koonin, Eugene V. ;

Makarova, Kira S. .

RNA BIOLOGY, 2013, 10 (05) :679-686

[12] CRISPR-Cas13a Nanomachine Based Simple Technology for Avian Influenza A (H7N9) Virus On-Site Detection [J].

Liu, Yufeng ;

Xu, Hongpan ;

Liu, Chang ;

Peng, Lijun ;

Khan, Haroon ;

Cui, Lunbiao ;

Huang, Rui ;

Wu, Chao ;

Shen, Sisi ;

Wang, Su ;

Liang, Wenbiao ;

Li, Zhiyang ;

Xu, Benbo ;

He, Nongyue .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2019, 15 (04) :790-798

[13] CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea [J].

Marraffini, Luciano A. ;

Sontheimer, Erik J. .

NATURE REVIEWS GENETICS, 2010, 11 (03) :181-190

[14]

Patterson JL, RAPID FULLY MICROFLU

[15] FIND Tuberculosis Strain Bank: a Resource for Researchers and Developers Working on Tests To Detect Mycobacterium tuberculosis and Related Drug Resistance [J].

Tessema, Belay ;

Nabeta, Pamela ;

Valli, Eloise ;

Albertini, Audrey ;

Collantes, Jimena ;

Lan, Nguyen Huu ;

Romancenco, Elena ;

Tukavdze, Nestani ;

Denkinger, Claudia M. ;

Dolinger, David L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (04) :1066-1073

[16] Tuberculosis: advances and challenges in development of new diagnostics and biomarkers [J].

Walzl, Gerhard ;

McNerney, Ruth ;

du Plessis, Nelita ;

Bates, Matthew ;

McHugh, Timothy D. ;

Chegou, Novel N. ;

Zumla, Alimuddin .

LANCET INFECTIOUS DISEASES, 2018, 18 (07) :E199-E210

[17]

World Health Organization, 2011, POL STAT AUT REAL TI

[18]

World Health Organization, 2018, GLOB TUB REP 2017

[19] Room Temperature Detection of Plasma Epstein-Barr Virus DNA with CRISPR-Cas13 [J].

Wu, Yetao ;

Liu, Shang-Xin ;

Wang, Fang ;

Zeng, Mu-Sheng .

CLINICAL CHEMISTRY, 2019, 65 (04) :591-592

[20] Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9 [J].

Wu, Yuxuan ;

Liang, Dan ;

Wang, Yinghua ;

Bai, Meizhu ;

Tang, Wei ;

Bao, Shiming ;

Yan, Zhiqiang ;

Li, Dangsheng ;

Li, Jinsong .

CELL STEM CELL, 2013, 13 (06) :659-662

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