Re-identification of the halotolerant, biosurfactant-producing Bacillus licheniformis strain JF-2 as Bacillus mojavensis strain JF-2

被引：23

作者：

Folmsbee, Martha ^{[1
]}

Duncan, Kathleen ^{[1
]}

Han, Sung Ok ^{[1
]}

Nagle, David ^{[1
]}

Jennings, Eleanor ^{[1
]}

McInerney, Michael ^{[1
]}

机构：

[1] Univ Oklahoma, Norman, OK 73019 USA

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2006年 / 29卷 / 08期

关键词：

Bacillus subtilis; Bacillus mojavensis; Bacillus licheniformis; phylogeny; anaerobic growth; DNA-DNA similarity; 16S rRNA gene;

D O I：

10.1016/j.syapm.2006.01.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensis(T) and not with B. licheniformis(T). DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensis(T) and only 11% similar to B. licheniformisT. Both strain JF-2 and B. mojavensisT required DNA for anaerobic growth, but B. licheniformis(T) did not. B. mojavensisT and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformis(T) grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis. (c) 2006 Elsevier GmbH. All rights reserved.

引用

页码：645 / 649

页数：5

共 19 条

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