Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato

被引：16

作者：

Karki, Hari S. ^{[1
]}

Halterman, Dennis A. ^{[1
]}

机构：

[1] USDA ARS, Madison, WI 53726 USA

来源：

BIO-PROTOCOL | 2021年 / 11卷 / 04期

关键词：

Phytophthora infestans; Potato; Detached leaf assay; Late blight; RESISTANCE; PLANT; GENE;

D O I：

10.21769/BioProtoc.3926

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the virulence assay of P. infestans in potato leaves. A late blight infection assay using a potato detached leaf is a semi-high throughput assay in which hundreds of plants can be screened in a laboratory setting.

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