Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery

被引:57
作者
Scholler, Nathalie
Garvik, Barbara
Hayden-Ledbetter, Martha
Kline, Toni
Urban, Nicole
机构
[1] Univ Washington, Translat Outcomes Res Lab, Fred Hutchinson Canc Res Ctr, Mol Diagnost Program, Seattle, WA 98109 USA
[2] Univ Washington, Trub Pharmaceut Inc, Seattle, WA 98195 USA
[3] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA
关键词
CA125; mesothelin; high-throughput screening; cell adhesion model system; ovarian cancer;
D O I
10.1016/j.canlet.2006.03.029
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. We finally demonstrated that a mesothelin chimeric protein and anti-CA125 antibodies block CA125/mesothelin-dependent cell attachment. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:130 / 136
页数:7
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