Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

被引：37

作者：

Chen, LQ ^{[1
]}

Jiang, HB

Zhou, ZL

Li, K

Deng, GY

Liu, ZJ

机构：

[1] E China Normal Univ, Dept Biol, Shanghai 200062, Peoples R China

[2] NuGen Technol Inc, San Carlos, CA 94070 USA

[3] Auburn Univ, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2004年 / 138卷 / 03期

基金：

中国国家自然科学基金;

关键词：

decapoda; developmental stages; enzyme-linked immunosorbent assay; Eriocheir sinensis; ovary; gel filtration chromatography; purification; vitellin;

D O I：

10.1016/j.cbpc.2004.04.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：305 / 311

页数：7

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