Immunogenicity of bacteriophage lambda particles displaying porcine Circovirus 2 (PCV2) capsid protein epitopes

被引：44

作者：

Gamage, Lakshman N. A. ^{[2
]}

Ellis, John ^{[2
]}

Hayes, Sidney ^{[1
]}

机构：

[1] Univ Saskatchewan, Coll Med, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada

[2] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Microbiol, Saskatoon, SK S7N 5E5, Canada

来源：

VACCINE | 2009年 / 27卷 / 47期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Phage display vaccine; Bacteriophage lambda; Porcine Circovirus 2; PCV2 ORF2 capsid protein; MULTISYSTEMIC WASTING SYNDROME; TYPE-2; PCV2; IMMUNE-RESPONSES; CELL-LINE; VACCINE; PIGS; PHAGE; EXPRESSION; ANTIBODIES; VIRUS;

D O I：

10.1016/j.vaccine.2009.08.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Phage lambda particles displaying four immunodominant regions of porcine Circovirus 2 (PCV2) capsid protein (LDP-D-CAP) was shown to be immunogenic in pigs. The immunodominant regions were fused to the carboxyl-terminal of lambda head protein D. Expression of D-CAP on lambda display particles was demonstrated by ELISA and Western blots. Pigs receiving LDP-D-CAP, without incorporating adjuvant, showed significant anti-PCV2 immune response following the primary vaccination. The LDP-D-CAP preparation induced PCV2 neutralizing antibodies. Delayed type hypersensitivity (DTH) reaction scores revealed that the immunized pigs were hypersensitive to both lambda Phaga and PCV2 antigens. The LDP-D-CAP elicited both cellular and humoral immune responses. Neither LDP-D-CAP nor the lambda control elicited any untoward local or systemic reactions following immunization. These studies produced the first potential phage vaccine to porcine Circovirus 2. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：6595 / 6604

页数：10

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