Chryseobacterium echinoideorum sp nov., isolated from sea urchins (Tripneustes gratilla)

A Gram-stain-negative, aerobic, non-motile, rod-shaped, flexirubin-producing bacterium, designated strain CC-CZW010(T), was isolated from the edible sea urchin Tripneustes gratilla in Penghu Island, Taiwan. The isolate grew optimally at pH 7.0 and 30 degrees C in the presence of 2% (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were Chryseobacterium taihuense NBRC 108747(T) (97.6 %) and Chryseobacterium aquaticum KCTC 12483(T) (96.7 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed a distinct taxonomic position attained by strain CC-CZW010(T) with respect to other species of the genus Chryseobacterium. Strain CC-CZW010(T) possessed iso-C-15 : 0, anteiso-C-15 : 0, iso-C-17 (: 0) 3-OH, summed feature 3 (comprising C-16 : 1 omega 7c/C-16 : 1 omega 6c) and summed feature 9 (comprising C-16 : 0 10-methyl/iso-C-17 : 1 omega 9c) as predominant fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified lipids and five aminolipids. The polyamine pattern contained the major compound sym-homospermidine. Menaquinone 6 (MK-6) was the predominant respiratory quinone, and the G + C content of the genomic DNA was 36.4 mol%. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-CZW010(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium echinoideorum sp. nov. is proposed. The type strain is CC-CZW010(T) (=BCRC 80786(T) =JCM 30470(T)).