Cytocines, produced by virtually all types of cells, are multifunctional polypeptide. TNF-alpha is a plethoric cytokine that has a major role in natural and acquired immune, cell regulation and differentiation and apoptosis. IL-6 has a lot of functions in natural and acquired immune, inhibit growth at carcinoma, tissue's growthing and differential. In this study, serum IL-6 and TNF-alpha levels were investigated in fibrosarcoma induced by 3-methylcholantrene. Sixteen male Spraque Dawley rats were used in this study. The experiment took about 150-210 days until the appearance of tumor tissue. 0.2mg 3-methylcholantrene solved in 0.25ml sesame oil were injected subcutaneously on the neck to experimental group, however only sesame oil given to control group. To find out whether any tumor tissue occurred animals were palpated daily. At the end of the experiment, the blood of animals was taken from atrium of the hearth under the ether anesthesia and than serum was prepared. Serum IL-6 and TNF-alpha levels were analyzed using BioSource kits at ELISA. Serum IL-6 levels were found 67.85+/-3.13 pg/ml in experimental group; 38.45+/-3.39 pg/ml in control group. It was 1.8 times higher than controls. Serum TNF-alpha levels were 53.35+/-9.08 pg/ml in experimental group; 26.42+/-2.22 pg/ml in control group. It was 2 times higher than controls. In the light of these findings it is thought that results of study are beneficial to understand pathogenesis and prognosis of cancer.
