Dietary intervention using (1,3)/(1,6)-β-glucan, a fungus-derived soluble prebiotic ameliorates high-fat diet-induced metabolic distress and alters beneficially the gut microbiota in mice model

被引：38

作者：

Muthuramalingam, Karthika ^{[1
]}

Singh, Vineet ^{[2
]}

Choi, Changmin ^{[1
]}

Choi, Seung In ^{[3
]}

Kim, Young Mee ^{[1
]}

Unno, Tatsuya ^{[2
,4
]}

Cho, Moonjae ^{[1
,5
,6
]}

机构：

[1] Jeju Natl Univ, Sch Med, Dept Biochem, Jeju 63243, South Korea

[2] Jeju Natl Univ, Fac Biotechnol, Sch Life Sci, SARI, Jeju 63243, South Korea

[3] Quegen Biotech Co Ltd, Dept Pharmaceut Res Inst, Seoul 429931, South Korea

[4] Jeju Natl Univ, Subtrop Trop Organism Gene Bank, Jeju 63243, South Korea

[5] Jeju Natl Univ, Inst Med Sci, Jeju 63241, South Korea

[6] Jeju Natl Univ, Interdisciplinary Grad Program Adv Convergence Te, Jeju 63241, South Korea

来源：

EUROPEAN JOURNAL OF NUTRITION | 2020年 / 59卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

High-fat diet; Gut microbiota; beta-glucan; Prebiotics; Obesity; Metabolic syndrome; RNA GENE DATABASE; INDUCED OBESITY; MOUSE MODEL; SENSITIVITY; DYSBIOSIS; BACTERIA; TRANSIT; IMPACT;

D O I：

10.1007/s00394-019-02110-5

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Purpose Western diet, rich in carbohydrates and fat, is said to be a major factor underlying metabolic syndrome. Interventions with prebiotics, the key modulators of the gut microbiota, have paramount impact on host-associated metabolic disorders. Herein, we investigated the effect of fungus-derived (1,3)/(1,6)-beta-glucan, a highly soluble dietary fiber, on high-fat diet (HFD)-induced metabolic distress. Methods Male C57BL/6 J mice were fed with different diet groups (n = 11): control diet, HFD, 3 g/kg or 5 g/kg of beta-glucan-incorporated HFD. At the end of experimental study period (12th week), body weight, feces weight and fecal moisture content were observed. Further, colonic motility was measured using activated charcoal meal study. Proteins extracted from liver and intestine tissues were subjected to western blot technique. Paraffin-embedded intestinal tissues were sectioned for histochemical [Periodic acid-Schiff (PAS) and Alcian blue (AB) staining] analysis. Fecal microbiota analysis was performed using MOTHUR bioinformatic software. Results beta-glucan consumption exhibited anti-obesity property in mice groups fed with HFD. In addition, beta-glucan ameliorated HFD-induced hepatic stress, colonic motility and intestinal atrophy (reduction in colon length, goblet cells, and mucosal layer thickness). Further, beta-glucan incorporation shifted bacterial community by increasing butyrate-producing bacteria such as Anaerostipes, Coprobacillus, and Roseburia and decreasing reportedly obesity-associated bacteria such as Parabacteroides and Lactococcus. Conclusion Altogether, the outcomes of this present pre-clinical animal study show beta-glucan to be a promising therapeutic candidate in the treatment of HFD-induced metabolic distress. Further comprehensive research has to be conducted to brace its clinical relevance, reproducibility and efficacy for aiding human health. [GRAPHICS] .

引用

页码：2617 / 2629

页数：13