Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry

被引:54
作者
Zhang, Hx
Hu, C. X.
Liu, C. P.
Li, H. F.
Wang, J. S.
Yuan, K. L.
Tang, J. W.
Xu, G. W. [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
[2] Dalian Med Univ, Dept Pathol, Dalian 116027, Peoples R China
基金
中国国家自然科学基金;
关键词
bioactive compounds; traditional Chinese medicines; cell extract; gas chromatography-mass spectrometer; murine ascites hepatocarcinoma cell; elemene emulsion injection; zedoary turmeric oil and glucose injection;
D O I
10.1016/j.jpba.2006.06.033
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
As the cost of drug development is always many times more than that of drug discovery, predictive methods aiding in the screening of bioavailable drug candidates are of profound significance. In this paper, a novel method for screening bioactive compounds from traditional Chinese medicines (TCMs) was developed by using living cell extract and gas chromatography (GC)-mass spectrometer (MS). The method was validated by using elemene emulsion injection (EEI), a typical TCM with known active compound, to interact with murine ascites hepatocarcinoma cell strain with high metastatic potential (HCa-F). Finally, the method was applied to screen the bioactive compounds from multi-component zedoary turmeric oil and glucose injection (ZTOGI). After HCa-F cells was incubated in ZTOGI, ethyl acetate (EtOAc) was used to extract the compounds in the cells for GC-MS analysis. Fourteen compounds were detected in the desorption eluate of HCa-F cell extract of ZTOGI, and further identified by MS. Curzerene and beta-elemene were found to be two major bioactive compounds in ZTOGI. These results show that the method developed may be applied to quickly screen the potential bioactive components in TCMs interacting with the target cells. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:151 / 157
页数:7
相关论文
共 41 条
[1]  
[Anonymous], 2000, HANDB EXP PHARM
[2]   pH-metric logP 10. Determination of liposomal membrane-water partition coefficients of ionizable drugs [J].
Avdeef, A ;
Box, KJ ;
Comer, JEA ;
Hibbert, C ;
Tam, KY .
PHARMACEUTICAL RESEARCH, 1998, 15 (02) :209-215
[3]   IMMOBILIZED-LIPOSOME CHROMATOGRAPHIC ANALYSIS OF DRUG PARTITIONING INTO LIPID BILAYERS [J].
BEIGI, F ;
YANG, Q ;
LUNDAHL, P .
JOURNAL OF CHROMATOGRAPHY A, 1995, 704 (02) :315-321
[4]   Immobilized liposome and biomembrane partitioning chromatography of drugs for prediction of drug transport [J].
Beigi, F ;
Gottschalk, I ;
Hägglund, CL ;
Haneskog, L ;
Brekkan, E ;
Zhang, YX ;
Österberg, T ;
Lundahl, P .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1998, 164 (1-2) :129-137
[5]  
CHEN G, 2000, J PHARM TOXICOL, V42, P199
[6]   Partition coefficients by curve fitting: The use of two different octanol volumes in a dual-phase potentiometric titration [J].
Clarke, FH ;
Cahoon, NM .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1996, 85 (02) :178-183
[7]  
DeVito SC, 2000, HANDBOOK OF PROPERTY ESTIMATION METHODS FOR CHEMICALS, P261
[8]   Hypothesis of potential active components in Angelica sinensis by using biomembrane extraction and high performance liquid chromatography [J].
Dong, ZB ;
Li, SP ;
Hong, A ;
Zhu, Q .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 38 (04) :664-669
[9]   Chemical and biological evaluation of the essential oils of different Melaleuca species [J].
Farag, RS ;
Shalaby, AS ;
El-Baroty, GA ;
Ibrahim, NA ;
Ali, MA ;
Hassan, EM .
PHYTOTHERAPY RESEARCH, 2004, 18 (01) :30-35
[10]   APPLICATION OF CHEMOMETRICALLY PROCESSED CHROMATOGRAPHIC DATA FOR PHARMACOLOGICALLY RELEVANT CLASSIFICATION OF ANTIHISTAMINE DRUGS [J].
GAMIYILINKOU, R ;
NASAL, A ;
KALISZAN, R .
JOURNAL OF CHROMATOGRAPHY, 1993, 633 (1-2) :57-63