Performance evaluation of an Indoxyl Sulfate Assay Kit "NIPRO"

被引：6

作者：

Fushimi, Yuki ^{[2
]}

Tatebe, Junko ^{[3
]}

Okuda, Yuko ^{[2
]}

Ishii, Toshiaki ^{[2
]}

Ujiie, Shinji ^{[2
]}

Morita, Toshisuke ^{[1
,2
]}

机构：

[1] Toho Univ, Dept Lab Med, Grad Sch Med, Ota Ku, 5-21-16 Omori Nishi, Tokyo 1438540, Japan

[2] Toho Univ, Dept Clin Lab, Omori Med Ctr, Tokyo, Japan

[3] Toho Univ, Dept Lab Med, Grad Sch Med, Tokyo, Japan

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2019年 / 57卷 / 11期

关键词：

analytical performance; cardiorenal syndrome; indoxyl sulfate; OXIDATIVE STRESS; P-CRESOL;

D O I：

10.1515/cclm-2019-0218

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The relationship between renal disease and cardiovascular disease (CVD) is currently known as cardiorenal syndrome. Indoxyl sulfate (IS) is one of the uremic toxins that accelerates the progression of cardiorenal syndrome. This report presents a new method for measuring IS in a simpler way. Methods: We evaluated the analytical performance of an IS Assay Kit "NIPRO" loaded on LABOSPECT 008. The evaluated analytical performances included accuracy, precision, dilution linearity, limit of detection (LOD), limit of quantitation (LOQ), recovery test, interference test and comparison against assays performed by high-performance liquid chromatography (HPLC). Results: Total precision showed a <5.3% coefficient of variation at IS concentrations of 3.57-277.73 mu mol/L, and satisfactory results were observed in the dilution linearity, LOD, LOQ, recovery and interference tests. The IS Assay Kit "NIPRO" showed a high correlation with the HPLC conventional method (r =0.993). Conclusions: The IS Assay Kit "NIPRO" demonstrated satisfactory analytical performance, and this suggests it could shortly become another common method to measure circulating IS.

引用

页码：1770 / 1776

页数：7

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