Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy

被引：4

作者：

Yamanaka, Yukito ^{[1
]}

Takenaka, Nana ^{[2
]}

Sakurai, Hidetoshi ^{[2
]}

Ueno, Morio ^{[1
]}

Kinoshita, Shigeru ^{[3
]}

Sotozono, Chie ^{[1
]}

Sato, Takahiko ^{[1
,4
,5
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Ophthalmol, Kyoto 6028566, Japan

[2] Kyoto Univ, Ctr IPS Cell Res & Applicat, Kyoto 6068507, Japan

[3] Kyoto Prefectural Univ Med, Dept Frontier Med Sci & Technol Ophthalmol, Kyoto 6028566, Japan

[4] Fujita Hlth Univ, Dept Anat, Toyoake, Aichi 4701192, Japan

[5] AMED CREST, Chiyoda Ku, AMED 1-7-1 Otemachi, Tokyo 1000004, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 14期

关键词：

muscle stem cell; orbicularis oculi; cell transplantation; Duchenne muscular dystrophy; SATELLITE CELL; UTROPHIN; PROTEIN; MARKER;

D O I：

10.3390/ijms20143456

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myogenic differentiation. In this context, we isolated CD56+CD82+ cells from the extra eyelid tissue of young and aged patients, and tested in vitro myogenic differentiation potential. In the current study, myogenic cells derived from extra eyelid tissue were characterized and compared with immortalized human myogenic cells. We found that myogenic cells derived from extra eyelid tissue proliferated and differentiated myofibers in vitro, and restored DYSTROPHIN or PAX7 expression after transplantation with these cells in mice with Duchenne muscular dystrophy. Thus, human myogenic cells derived from extra eyelid tissue including the orbicularis oculi might be good candidates for stem cell-based therapies for treating muscular diseases.

引用

页数：11

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