Live-Cell Pyrophosphate Imaging by in Situ Hot-Spot Generation

被引：40

作者：

Li, Mingmin ^{[1
,2
]}

Li, Jin ^{[1
,2
]}

Di, Huixia ^{[1
,2
]}

Liu, Huiqiao ^{[1
,2
]}

Liu, Dingbin ^{[1
,2
,3
]}

机构：

[1] Nankai Univ, State Key Lab Med Chem Biol, Res Ctr Analyt Sci, Coll Chem, Tianjin 300071, Peoples R China

[2] Nankai Univ, Tianjin Key Lab Mol Recognit & Biosensing, Tianjin 300071, Peoples R China

[3] Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 06期

基金：

中国国家自然科学基金;

关键词：

SELECTIVE FLUORESCENT CHEMOSENSOR; PLASMONIC NANOPARTICLES; ALKALINE-PHOSPHATASE; ANION RECOGNITION; GOLD; COMPLEXES; DNA; COORDINATION; NANOPROBES; METABOLISM;

D O I：

10.1021/acs.analchem.6b04786

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Controlling the electromagnetic hot-spot generation is essential for surface-enhanced Raman scattering (SERS) assays. Current hot-spot-based SERS assays have been extensively studied in solutions or on substrates. However, probing biospecies by controlling the hot-spot assembly in living systems has not been demonstrated thus far. Herein, we report a background -free SERS probe for imaging pyrophosphate (PPi), a biochemically significant anion, in living cells. Intracellular PPi is able to induce the nanoparticle dimerization, thus creating an intense electromagnetic hot spot and dramatically enhancing the signal of the Raman reporters residing in the hot spot. More impressively, the reporter we used in this study provides a strong and sharp single peak in the cellular Raman-silent region (1800-2800 cm(-1)), thus eliminating the possible background interference. This strategy could be readily extended to detect other biomarkers by only replacing the recognition ligands.

引用

页码：3532 / 3537

页数：6

共 38 条

[1] Naphthalene Carbohydrazone Based Dizinc(II) Chemosensor for a Pyrophosphate Ion and Its DNA Assessment Application in Polymerase Chain Reaction Products [J].