Molecular cloning and sequence analysis of the DnaA gene of Staphylococcus aureus

被引：0

作者：

Katayama, H ^{[1
]}

Mizushima, T ^{[1
]}

Miki, T ^{[1
]}

Sekimizu, K ^{[1
]}

机构：

[1] KYUSHU UNIV, FAC PHARMACEUT SCI, FUKUOKA 81282, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1997年 / 20卷 / 07期

关键词：

DnaA protein; Staphylococcus aureus; molecular cloning;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The dnaA gene of Staphylococcus aureus has now been cloned and sequenced The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.

引用

页码：820 / 822

页数：3

共 24 条

[1] NUCLEOTIDE-SEQUENCE OF THE RECF GENE-CLUSTER FROM STAPHYLOCOCCUS-AUREUS AND COMPLEMENTATION ANALYSIS IN BACILLUS-SUBTILIS RECF MUTANTS [J].

ALONSO, JC ;

FISHER, LM .

MOLECULAR & GENERAL GENETICS, 1995, 246 (06) :680-686

[2] DUPLEX OPENING BY DNAA PROTEIN AT NOVEL SEQUENCES IN INITIATION OF REPLICATION AT THE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME [J].

BRAMHILL, D ;

KORNBERG, A .

CELL, 1988, 52 (05) :743-755

[3] CONSERVED GENE ARRANGEMENT IN THE ORIGIN REGION OF THE STREPTOMYCES-COELICOLOR CHROMOSOME [J].

CALCUTT, MJ ;

SCHMIDT, FJ .

JOURNAL OF BACTERIOLOGY, 1992, 174 (10) :3220-3226

[4]

CASTUMA CE, 1993, J BIOL CHEM, V268, P24665

[5] STRUCTURE OF THE DNAA REGION OF PSEUDOMONAS-PUTIDA - CONSERVATION AMONG 3 BACTERIA, BACILLUS-SUBTILIS, ESCHERICHIA-COLI AND PSEUDOMONAS-PUTIDA [J].

FUJITA, MQ ;

YOSHIKAWA, H ;

OGASAWARA, N .

MOLECULAR & GENERAL GENETICS, 1989, 215 (03) :381-387

[6] STRUCTURE OF THE DNAA AND DNAA-BOX REGION IN THE MYCOPLASMA-CAPRICOLUM CHROMOSOME - CONSERVATION AND VARIATIONS IN THE COURSE OF EVOLUTION [J].

FUJITA, MQ ;

YOSHIKAWA, H ;

OGASAWARA, N .

GENE, 1992, 110 (01) :17-23

[7] THE DNAA PROTEIN COMPLEX WITH THE ESCHERICHIA-COLI CHROMOSOMAL REPLICATION ORIGIN (ORIC) AND OTHER DNA SITES [J].

FULLER, RS ;

FUNNELL, BE ;

KORNBERG, A .

CELL, 1984, 38 (03) :889-900

[8] Membrane regulation of the chromosomal replication activity of E-coli DnaA requires a discrete site on the protein [J].

Garner, J ;

Crooke, E .

EMBO JOURNAL, 1996, 15 (09) :2313-2321

[9]

KATAYAMA T, 1994, J BIOL CHEM, V269, P12698

[10]

KATAYAMA T, 1994, J BIOL CHEM, V269, P22075

← 1 2 3 →