Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity

被引:91
作者
Christelova, Pavla [1 ]
De langhe, Edmond [2 ]
Hribova, Eva [1 ]
Cizkova, Jana [1 ]
Sardos, Julie [3 ]
Husakova, Marketa [1 ]
Van den Houwe, Ines [4 ]
Sutanto, Agus [5 ]
Kepler, Angela Kay [6 ]
Swennen, Rony [2 ,4 ,7 ]
Roux, Nicolas [3 ]
Dolezel, Jaroslav [1 ]
机构
[1] Inst Expt Bot, Ctr Reg Hana Biotechnol & Agr Res, Slechtitelu 31, Olomouc 78371, Czech Republic
[2] Katholieke Univ Leuven, Lab Trop Crop Improvement, Div Crop Biotech, Willem Croylaan 42, B-3001 Heverlee, Belgium
[3] Biovers Int, Parc Sci Agropolis 2, F-34397 Montpellier, France
[4] Biovers Int, Willem Croylaan 42, B-3001 Heverlee, Belgium
[5] Indonesian Agr Agcy Res & Dev, JI Ragunan 29, Jakarta, Indonesia
[6] Hawaii & Maui Invas Species Comm, POB 983, Makawao, HI 96768 USA
[7] Int Inst Trop Agr, POB 10, Arusha, Tanzania
关键词
Classification; Gene bank; Genetic diversity; ITC collection; Microsatellites; Musa; GENETIC-LINKAGE MAP; BALBISIANA COLLA; BLACK SIGATOKA; POPULATION-STRUCTURE; ACUMINATA COLLA; PLANTAIN MUSA; GENOME; SPP; SSR; HYBRIDS;
D O I
10.1007/s10531-016-1273-9
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Bananas (Musa spp.) are one of the main fruit crops grown worldwide. With the annual production reaching 144 million tons, their production represents an important contribution to the economies of many countries in Asia, Africa, Latin-America and Pacific Islands. Most importantly, bananas are a staple food for millions of people living in the tropics. Unfortunately, sustainable banana production is endangered by various diseases and pests, and the breeding for resistant cultivars relies on a far too small base of genetic variation. Greater diversity needs to be incorporated in breeding, especially of wild species. Such work requires a large and thoroughly characterized germplasm collection, which also is a safe depository of genetic diversity. The largest ex situ Musa germplasm collection is kept at the International Transit Centre (ITC) in Leuven (Belgium) and currently comprises over 1500 accessions. This report summarizes the results of systematic cytological and molecular characterization of the Musa ITC collection. By December 2015, 630 accessions have been genotyped. The SSR markers confirmed the previous morphological based classification for 84% of ITC accessions analyzed. The remaining 16% of the genotyped entries may need field verification by taxonomist to decide if the unexpected classification by SSR genotyping was correct. The ploidy level estimation complements the molecular data. The genotyping continues for the entire ITC collection, including newly introduced accessions, to assure that the genotype of each accession is known in the largest global Musa gene bank.
引用
收藏
页码:801 / 824
页数:24
相关论文
共 89 条
[1]  
[Anonymous], CURRENT PROTOCOLS CY
[2]  
ARGENT G C G, 1976, Notes from the Royal Botanic Garden Edinburgh, V35, P77
[3]   Nuclear genome size and genomic distribution of ribosomal DNA in Musa and Ensete (Musaceae):: taxonomic implications [J].
Bartos, J ;
Alkhimova, O ;
Dolezelová, M ;
De Langhe, E ;
Dolezel, J .
CYTOGENETIC AND GENOME RESEARCH, 2005, 109 (1-3) :50-57
[4]   Identification of cytoplasmic ancestor gene-pools of Musa acuminata Colla and Musa balbisiana Colla and their hybrids by chloroplast and mitochondrial haplotyping [J].
Boonruangrod, R. ;
Desai, D. ;
Fluch, S. ;
Berenyi, M. ;
Burg, K. .
THEORETICAL AND APPLIED GENETICS, 2008, 118 (01) :43-55
[5]  
BOTSTEIN D, 1980, AM J HUM GENET, V32, P314
[6]   Ascertaining maternal and paternal lineage within Musa by chloroplast and mitochondrial DNA RFLP analyses [J].
Carreel, F ;
de Leon, DG ;
Lagoda, P ;
Lanaud, C ;
Jenny, C ;
Horry, JP ;
du Montcel, HT .
GENOME, 2002, 45 (04) :679-692
[7]   The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre [J].
Chase, R. ;
Sardos, J. ;
Ruas, M. ;
Van den Houwe, I. ;
Roux, N. ;
Hribova, E. ;
Dolezel, J. .
XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): IX INTERNATIONAL SYMPOSIUM ON BANANA: ISHS-PROMUSA SYMPOSIUM ON UNRAVELLING THE BANANA'S GENOMIC POTENTIAL, 2016, 1114 :61-65
[8]  
Cheesman E. E., 1947, Kew Bulletin, P97, DOI 10.2307/4109206
[9]   Maize simple repetitive DNA sequences: Abundance and allele variation [J].
Chin, ECL ;
Senior, ML ;
Shu, H ;
Smith, JSC .
GENOME, 1996, 39 (05) :866-873
[10]   A platform for efficient genotyping in Musa using microsatellite markers [J].
Christelova, Pavla ;
Valarik, Miroslav ;
Hribova, Eva ;
Van den houwe, Ines ;
Channeliere, Stephanie ;
Roux, Nicolas ;
Dolezel, Jaroslav .
AOB PLANTS, 2011,