A calmodulin-gated calcium channel links pathogen patterns to plant immunity

被引：387

作者：

Tian, Wang ^{[1
]}

Hou, Congcong ^{[1
]}

Ren, Zhijie ^{[2
]}

Wang, Chao ^{[1
]}

Zhao, Fugeng ^{[3
]}

Dahlbeck, Douglas ^{[1
]}

Hu, Songping ^{[1
]}

Zhang, Liying ^{[2
]}

Niu, Qi ^{[2
]}

Li, Legong ^{[2
]}

Staskawicz, Brian J. ^{[1
]}

Luan, Sheng ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[2] Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China

[3] Nanjing Univ, Coll Life Sci, Nanjing Univ Nanjing Forestry Univ Joint Inst Pla, Nanjing, Jiangsu, Peoples R China

来源：

NATURE | 2019年 / 572卷 / 7767期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

ION-CHANNEL; HYPERSENSITIVE RESPONSE; CYTOPLASMIC KINASE; ARABIDOPSIS; RECEPTOR; DEFENSE; GENE; COMPLEX; PHOSPHORYLATES; MICROBE;

D O I：

10.1038/s41586-019-1413-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pathogen-associated molecular patterns (PAMPs) activate innate immunity in both animals and plants. Although calcium has long been recognized as an essential signal for PAMP-triggered immunity in plants, the mechanism of PAMP-induced calcium signalling remains unknown(1,2). Here we report that calcium nutrient status is critical for calcium-dependent PAMP-triggered immunity in plants. When calcium supply is sufficient, two genes that encode cyclic nucleotide-gated channel (CNGC) proteins, CNGC2 and CNGC4, are essential for PAMP-induced calcium signalling in Arabidopsis(3-7). In a reconstitution system, we find that the CNGC2 and CNGC4 proteins together-but neither alone-assemble into a functional calcium channel that is blocked by calmodulin in the resting state. Upon pathogen attack, the channel is phosphorylated and activated by the effector kinase BOTRYTIS-INDUCED KINASE1 (BIK1) of the pattern-recognition receptor complex, and this triggers an increase in the concentration of cytosolic calcium8-10. The CNGC-mediated calcium entry thus provides a critical link between the pattern-recognition receptor complex and calcium-dependent immunity programs in the PAMP-triggered immunity signalling pathway in plants.

引用

页码：131 / +

页数：20

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