A calmodulin-gated calcium channel links pathogen patterns to plant immunity

被引：387

作者：

Tian, Wang ^{[1
]}

Hou, Congcong ^{[1
]}

Ren, Zhijie ^{[2
]}

Wang, Chao ^{[1
]}

Zhao, Fugeng ^{[3
]}

Dahlbeck, Douglas ^{[1
]}

Hu, Songping ^{[1
]}

Zhang, Liying ^{[2
]}

Niu, Qi ^{[2
]}

Li, Legong ^{[2
]}

Staskawicz, Brian J. ^{[1
]}

Luan, Sheng ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[2] Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China

[3] Nanjing Univ, Coll Life Sci, Nanjing Univ Nanjing Forestry Univ Joint Inst Pla, Nanjing, Jiangsu, Peoples R China

来源：

NATURE | 2019年 / 572卷 / 7767期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

ION-CHANNEL; HYPERSENSITIVE RESPONSE; CYTOPLASMIC KINASE; ARABIDOPSIS; RECEPTOR; DEFENSE; GENE; COMPLEX; PHOSPHORYLATES; MICROBE;

D O I：

10.1038/s41586-019-1413-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pathogen-associated molecular patterns (PAMPs) activate innate immunity in both animals and plants. Although calcium has long been recognized as an essential signal for PAMP-triggered immunity in plants, the mechanism of PAMP-induced calcium signalling remains unknown(1,2). Here we report that calcium nutrient status is critical for calcium-dependent PAMP-triggered immunity in plants. When calcium supply is sufficient, two genes that encode cyclic nucleotide-gated channel (CNGC) proteins, CNGC2 and CNGC4, are essential for PAMP-induced calcium signalling in Arabidopsis(3-7). In a reconstitution system, we find that the CNGC2 and CNGC4 proteins together-but neither alone-assemble into a functional calcium channel that is blocked by calmodulin in the resting state. Upon pathogen attack, the channel is phosphorylated and activated by the effector kinase BOTRYTIS-INDUCED KINASE1 (BIK1) of the pattern-recognition receptor complex, and this triggers an increase in the concentration of cytosolic calcium8-10. The CNGC-mediated calcium entry thus provides a critical link between the pattern-recognition receptor complex and calcium-dependent immunity programs in the PAMP-triggered immunity signalling pathway in plants.

引用

页码：131 / +

页数：20

共 35 条

[1] Death don't have no mercy and neither does calcium:: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and innate immunity [J].

Ali, Rashid ;

Ma, Wei ;

Lemtiri-Chlieh, Fouad ;

Tsaltas, Dimitrios ;

Leng, Qiang ;

von Bodman, Susannne ;

Berkowitz, Gerald A. .

PLANT CELL, 2007, 19 (03) :1081-1095

[2] A high-affinity calmodulin-binding site in a tobacco plasma-membrane channel protein coincides with a characteristic element of cyclic nucleotide-binding domains [J].

Arazi, T ;

Kaplan, B ;

Fromm, H .

PLANT MOLECULAR BIOLOGY, 2000, 42 (04) :591-601

[3] HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family [J].

Balagué, C ;

Lin, BQ ;

Alcon, C ;

Flottes, G ;

Malmström, S ;

Köhler, C ;

Neuhaus, G ;

Pelletier, G ;

Gaymard, F ;

Roby, D .

PLANT CELL, 2003, 15 (02) :365-379

[4] To die or not to die? Lessons from lesion mimic mutants [J].

Bruggeman, Quentin ;

Raynaud, Cecile ;

Benhamed, Moussa ;

Delarue, Marianne .

FRONTIERS IN PLANT SCIENCE, 2015, 6 :1-22

[5] A cyclic nucleotide-gated ion channel, CNGC2, is crucial for plant development and adaptation to calcium stress [J].

Chan, CWM ;

Schorrak, LM ;

Smith, RK ;

Bent, AF ;

Sussman, MR .

PLANT PHYSIOLOGY, 2003, 132 (02) :728-731

[6] The Arabidopsis Cyclic Nucleotide-Gated Ion Channels AtCNGC2 and AtCNGC4 Work in the Same Signaling Pathway to Regulate Pathogen Defense and Floral Transition [J].

Chin, Kimberley ;

DeFalco, Thomas A. ;

Moeder, Wolfgang ;

Yoshioka, Keiko .

PLANT PHYSIOLOGY, 2013, 163 (02) :611-624

[7] A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence [J].

Chinchilla, Delphine ;

Zipfel, Cyril ;

Robatzek, Silke ;

Kemmerling, Birgit ;

Nuernberger, Thorsten ;

Jones, Jonathan D. G. ;

Felix, Georg ;

Boller, Thomas .

NATURE, 2007, 448 (7152) :497-U12

[8] Calcium signaling [J].

Clapham, David E. .

CELL, 2007, 131 (06) :1047-1058

[9] The Arabidopsis dnd1 "defense, no death" gene encodes a mutated cyclic nucleotide-gated ion channel [J].

Clough, SJ ;

Fengler, KA ;

Yu, IC ;

Lippok, B ;

Smith, RK ;

Bent, AF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) :9323-9328

[10] Cell-Specific Vacuolar Calcium Storage Mediated by CAX1 Regulates Apoplastic Calcium Concentration, Gas Exchange, and Plant Productivity in Arabidopsis [J].

Conn, Simon J. ;

Gilliham, Matthew ;

Athman, Asmini ;

Schreiber, Andreas W. ;

Baumann, Ute ;

Moller, Isabel ;

Cheng, Ning-Hui ;

Stancombe, Matthew A. ;

Hirschi, Kendal D. ;

Webb, Alex A. R. ;

Burton, Rachel ;

Kaiser, Brent N. ;

Tyerman, Stephen D. ;

Leigh, Roger A. .

PLANT CELL, 2011, 23 (01) :240-257

← 1 2 3 4 →