Field confinement with aberration correction for solid immersion lens based fluorescence correlation spectroscopy

被引：5

作者：

Rao, Ramachandra ^{[1
]}

Mitic, Jelena ^{[1
]}

Serov, Alexandre ^{[1
]}

Leitgeb, Rainer A. ^{[1
]}

Lasser, Theo ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Opt Biomed, CH-1015 Lausanne, Switzerland

来源：

OPTICS COMMUNICATIONS | 2007年 / 271卷 / 02期

关键词：

fluorescence correlation spectroscopy; solid immersion lens; aberration compensation;

D O I：

10.1016/j.optcom.2006.10.044

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The solid immersion lens (SIL) as a tool for increasing the field confinement as well as providing optimal performance by aberration compensation in a confocall fluorescence correlation spectroscopy (FCS) system is illustrated here. Using Zernike polynomials we show that aberration compensation and the resultant pre-shaping of the incident wavefront enables near diffraction-limited performance. This is explained based on vectorial computations for high apertures in the Debye approximation. The obtained axial resolution parameters are compared with the obtained diffusion times in a SIL-FCS experiment for measurements in solutions done at the single molecule level. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：462 / 469

页数：8

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