Spermatozoa capture HIV-1 through heparan sulfate and efficiently transmit the virus to dendritic cells

被引：86

作者：

Ceballos, Ana ^{[1
]}

Remes Lenicov, Federico ^{[1
]}

Sabatte, Juan ^{[1
]}

Rodriguez Rodrigues, Christian ^{[1
]}

Cabrini, Mercedes ^{[1
]}

Jancic, Carolina ^{[2
]}

Raiden, Silvina ^{[2
]}

Donaldson, Monica ^{[3
]}

Agustin Pasqualini, Rodolfo, Jr. ^{[3
]}

Marin-Briggiler, Clara ^{[4
]}

Vazquez-Levin, Monica ^{[4
]}

Capani, Francisco ^{[1
]}

Amigorena, Sebastian ^{[5
]}

Geffner, Jorge ^{[1
,2
]}

机构：

[1] Univ Buenos Aires, Ctr Nacl Referencia SIDA, Fac Med, Buenos Aires, DF, Argentina

[2] Acad Nacl Med Buenos Aires, Inst Invest Hematol, Buenos Aires, DF, Argentina

[3] Inst Med Halitus, Buenos Aires, DF, Argentina

[4] Inst Biol & Med Expt, RA-1428 Buenos Aires, DF, Argentina

[5] Inst Curie, Inst Natl Sante & Rech Med U365, F-75248 Paris, France

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 2009年 / 206卷 / 12期

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; ENVELOPE GLYCOPROTEIN GP120; POLYMERASE-CHAIN-REACTION; VAGINAL EPITHELIAL-CELLS; HUMAN UTERINE CERVIX; CD4(+) T-CELLS; SEXUAL TRANSMISSION; DC-SIGN; MANNOSE RECEPTOR; SEMINAL PLASMA;

D O I：

10.1084/jem.20091579

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Semen is the main vector for HIV-1 dissemination worldwide. It contains three major sources of infectious virus: free virions, infected leukocytes, and spermatozoa-associated virions. We focused on the interaction of HIV-1 with human spermatozoa and dendritic cells (DCs). We report that heparan sulfate is expressed in spermatozoa and plays an important role in the capture of HIV-1. Spermatozoa-attached virus is efficiently transmitted to DCs, macrophages, and T cells. Interaction of spermatozoa with DCs not only leads to the transmission of HIV-1 and the internalization of the spermatozoa but also results in the phenotypic maturation of DCs and the production of IL-10 but not IL-12p70. At low values of extracellular pH (similar to 6.5 pH units), similar to those found in the vaginal mucosa after sexual intercourse, the binding of HIV-1 to the spermatozoa and the consequent transmission of HIV-1 to DCs were strongly enhanced. Our observations support the notion that far from being a passive carrier, spermatozoa acting in concert with DCs might affect the early course of sexual transmission of HIV-1 infection.

引用

页码：2717 / 2733

页数：17

共 85 条

[1]

[Anonymous], 1999, WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction

[2] Human immunodeficiency virus type 1 enters primary human brain microvascular endothelial cells by a mechanism involving cell surface proteoglycans independent of lipid rafts [J].

Argyris, EG ;

Acheampong, E ;

Nunnari, G ;

Mukhtar, M ;

Williams, KJ ;

Pomerantz, RJ .

JOURNAL OF VIROLOGY, 2003, 77 (22) :12140-12151

[3] HIV-PARTICLES IN SPERMATOZOA OF PATIENTS WITH AIDS AND THEIR TRANSFER INTO THE OOCYTE [J].

BACCETTI, B ;

BENEDETTO, A ;

BURRINI, AG ;

COLLODEL, G ;

CECCARINI, EC ;

CRISA, N ;

DICARO, A ;

ESTENOZ, M ;

GARBUGLIA, AR ;

MASSACESI, A ;

PIOMBONI, P ;

RENIERI, T ;

SOLAZZO, D .

JOURNAL OF CELL BIOLOGY, 1994, 127 (04) :903-914

[4] The debate on the presence of HIV-1 in human gametes [J].

Baccetti, B ;

Benedetto, A ;

Collodel, G ;

di Caro, A ;

Garbuglia, AR ;

Piomboni, P .

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1998, 41 (1-2) :41-67

[5] DETECTION OF HIV-1 PROVIRAL DNA IN SPERM FROM HIV-1-INFECTED MEN [J].

BAGASRA, O ;

FARZADEGAN, H ;

SESHAMMA, T ;

OAKES, JW ;

SAAH, A ;

POMERANTZ, RJ .

AIDS, 1994, 8 (12) :1669-1674

[6] Identification of CD4-independent HIV receptors on spermatozoa [J].

Bandivdekar, AH ;

Velhal, SM ;

Raghavan, VP .

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2003, 50 (04) :322-327

[7] Use of atomic force microscopy to reveal sperm ultrastructure in HIV-patients on highly active antiretroviral therapy [J].

Barboza, JM ;

Medina, H ;

Doria, M ;

Rivero, L ;

Hernandez, L ;

Joshi, NV .

ARCHIVES OF ANDROLOGY, 2004, 50 (02) :121-129

[8] HEAD-SPECIFIC MANNOSE LIGAND-RECEPTOR EXPRESSION IN HUMAN SPERMATOZOA IS DEPENDENT ON CAPACITATION-ASSOCIATED MEMBRANE CHOLESTEROL LOSS [J].

BENOFF, S ;

HURLEY, I ;

COOPER, GW ;

MANDEL, FS ;

ROSENFELD, DL ;

HERSHLAG, A .

HUMAN REPRODUCTION, 1993, 8 (12) :2141-2154

[9] Seminal plasma promotes the attraction of Langerhans cells via the secretion of CCL20 by vaginal epithelial cells:: involvement in the sexual transmission of HIV [J].

Berlier, W ;

Cremel, M ;

Hamzeh, H ;

Lévy, R ;

Lucht, F ;

Bourlet, T ;

Pozzetto, B ;

Delézay, O .

HUMAN REPRODUCTION, 2006, 21 (05) :1135-1142

[10] Heparan sulphate proteoglycans fine-tune mammalian physiology [J].

Bishop, Joseph R. ;

Schuksz, Manuela ;

Esko, Jeffrey D. .

NATURE, 2007, 446 (7139) :1030-1037

← 1 2 3 4 5 6 7 8 9 →