Nonlabens aestuariivivens sp nov., isolated from a tidal flat

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-31(T), was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-31(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 1.0-2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-31(T) belonged to the genus Nonlabens, clustering with the type strains of Nonlabens dokdonensis and Nonlabens xylanidelens, sharing 98.1% sequence similarity with each. Strain OITF-31(T) exhibited 16S rRNA gene sequence similarities of 93.9-95.8% to the type strains of other Nonlabens species and less than of 91.8% to the type strains of other recognized species. Strain OITF-31(T) contained MK-6 as the predominant menaquinone and iso-C-17:0 3-OH and iso-C-15:0 as the major fatty acids. The major polar lipids detected in strain OITF-31(T) were phosphatidylethanolamine, six unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain OITF-31(T) was 38.1 mol% and its mean DNA-DNA relatedness values with the type strains of N. dokdonensis and N. xylanidelens were 24 and 19 %, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-31(T) is separated from recognized species of the genus Nonlabens. On the basis of the data presented, strain OITF-31(T) is considered to represent a novel species of the genus Nonlabens, for which the name Nonlabens aestuariivivens sp. nov. is proposed. The type strain is OITF-31(T) (= KCTC 52502(T) =NBRC 112459(T)).