LC-MS/MS determination of tranexamic acid in human plasma after phospholipid clean-up

被引：13

作者：

Fabresse, Nicolas ^{[1
,2
]}

Fall, Fanta ^{[1
]}

Etting, Isabelle ^{[1
,2
]}

Devillier, Philippe ^{[3
,4
]}

Alvarez, Jean-Claude ^{[1
,2
]}

Grassin-Delyle, Stanislas ^{[1
,3
]}

机构：

[1] Univ Paris Saclay, Univ Versailles St Quentin, INSERM UMR 1173, Plateforme Spectrometrie Masse MasSpecLab,UFR Sci, Montigny Le Bretonneux, France

[2] Hop Raymond Poincare, AP HP, Serv Pharmacol Toxicol, Garches, France

[3] Hop Foch, Dept Malad Resp, Suresnes, France

[4] Univ Paris Saclay, Hop Foch, Univ Versailles St Quentin, Lab Pharmacol UPRES EA220, Suresnes, France

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2017年 / 141卷

关键词：

Tranexamic acid; Liquid chromatography; Mass spectrometry; Human plasma; Phospholipid removal; EMA guideline; SOLID-PHASE MICROEXTRACTION; CARDIOPULMONARY BYPASS; CARDIAC-SURGERY; POPULATION PHARMACOKINETICS; FIBRINOLYSIS; BIOANALYSIS; CHILDREN; BIOAVAILABILITY; INHIBITION; VOLUNTEERS;

D O I：

10.1016/j.jpba.2017.04.024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Tranexamic acid is a widely used antifibrinolytic drug but its pharmacology and pharmacokinetics remains poorly understood. Owing to the recent knowledge on phospholipid-induced matrix effects during human plasma analysis, our aim was to develop a liquid chromatography-mass spectrometry method for the quantitation of tranexamic acid after efficient sample clean-up. Sample preparation consisted in phospholipid removal and protein precipitation. Hydrophilic interaction liquid chromatography was used and the detection was achieved with multiple reaction monitoring. The method was validated according to the European Medicine Agency guideline in the range 1.0-1000.0 mu g/mL. The performance of the method was excellent with a precision in the range 1.2-3.0%, an accuracy between 88.4 and 96.6% and a coefficient of variation of the internal standard-normalized matrix factor below 6.7%. This method is suitable for the quantification of tranexamic acid in the wide range of concentrations observed during clinical studies, with all the advantages related to phospholipid removal. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：149 / 156

页数：8

共 36 条

[1] PHARMACOKINETICS OF TRANEXAMIC ACID IN PATIENTS WITH ULCERATIVE-COLITIS AND IN HEALTHY-VOLUNTEERS AFTER THE SINGLE INSTILLATION OF 2G RECTALLY [J].