Whole-genome sequencing reveals Listeria monocytogenes diversity and allows identification of long-term persistent strains in Brazil

被引：37
作者：
Camargo, Anderson C. [1 ]
Moura, Alexandra [2 ,3 ]
Avillan, Johannetsy [4 ]
Herman, Nicole [5 ]
McFarland, Adelle P. [6 ,9 ]
Sreevatsan, Srinand [7 ]
Call, Douglas R. [4 ]
Woodward, Joshua J. [6 ]
Lecuit, Marc [2 ,3 ,8 ]
Nero, Luis A. [1 ]
机构：
[1] Univ Fed Vicosa, Dept Vet, Vicosa, MG, Brazil
[2] Inst Pasteur, Biol Infect Unit, Paris, France
[3] INSERM, U1117, Paris, France
[4] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
[5] Univ Minnesota, Vet Populat Med Dept, St Paul, MN 55108 USA
[6] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[7] Michigan State Univ, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA
[8] Univ Paris, Necker Enfants Malades Univ Hosp, AP HP, Dept Infect Dis & Trop Med,Inst Imagine, Paris, France
[9] Washington Univ, Dept Pathol & Immunol, St Louis, MO 63110 USA
来源：
ENVIRONMENTAL MICROBIOLOGY | 2019年 / 21卷 / 12期
关键词：
SURVEILLANCE; INTERNALIN; TOOL; POLYMORPHISM; CONTRIBUTES; EVOLUTION; SEROVARS; DATABASE; CLONES; GENES;
D O I：
10.1111/1462-2920.14726
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Advances in whole-genome sequencing (WGS) technologies have documented genetic diversity and epidemiology of the major foodborne pathogen Listeria monocytogenes (Lm) in Europe and North America, but data concerning South America are scarce. Here, we examined the population structure and genetic diversity of this major foodborne pathogen collected in Brazil. Based on core genome multilocus sequence typing (cgMLST), isolates from lineages I (n = 22; 63%) and II (n = 13; 37%) were distributed into 10 different sublineages (SLs) and represented 31 new cgMLST types (CTs). The most prevalent SLs were SL9 (n = 9; 26%), SL3 (n = 6; 17%) and SL2 and SL218 (n = 5; 14%). Isolates belonging to CTs L2-SL9-ST9-CT4420 and L1-SL315-ST520-CT4429 were collected 3 and 9 years apart, respectively, revealing long-term persistence of Lm in Brazil. Genetic elements associated with stress survival were present in 60% of isolates (57% SSI-1 and 3% SSI-2). Pathogenic islands were present in 100% (LIPI-1), 43% (LIPI-3) and 6% (LIPI-4) of the isolates. Mutations leading to premature stop codons were detected in the prfA and inlA virulence genes. This study is an important contribution to understanding the genomic diversity and epidemiology of Lm in South America. In addition, the results highlight the importance of using WGS to reveal Lm long-term persistence.
引用
收藏
页码：4478 / 4487
页数：10
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