Near-infrared ratio fluorescent sensor for the study of PGP-1 in inflammation and tumor mice

被引：10

作者：

Cao, Ting ^{[1
]}

Zhang, Liang ^{[2
]}

Ma, Hong ^{[1
]}

Zheng, Lei ^{[1
]}

Cao, Yuping ^{[1
]}

Wang, Jiemin ^{[1
]}

Yang, Yuexia ^{[1
]}

Zhang, Jin ^{[1
]}

Qin, Wenwu ^{[1
]}

Liu, Yun ^{[2
]}

机构：

[1] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, State Key Lab Appl Organ Chem,Key Lab Special Fun, Lanzhou 730000, Peoples R China

[2] Army Med Univ, Xinqiao Hosp, Dept Radiol, Chongqing 400037, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 338卷

关键词：

Pyroglutamate aminopeptidase 1; Near-infrared ratio fluorescence probe; Drug induced; Inflammatory factor; Tumor imaging; PYRROLIDONECARBOXYLATE PEPTIDASE; PYROGLUTAMATE AMINOPEPTIDASE; PROBE; PURIFICATION; MECHANISMS; BIOLOGY; CELLS; SERUM; ACID;

D O I：

10.1016/j.snb.2021.129841

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Studies have found that there is overexpression of pyroglutamate aminopeptidase 1 (PGP-1) during inflammation, but the relationship between PGP-1 and disease is still unclear. To explore this situation, we designed the first near-infrared ratiometric fluorescence probe TP-1 for the PGP-1 specific detection to discuss the above problem. The large Stokes shift (?abs = 400 nm, ?em = 564 nm), high sensitivity and the lowest detection limit (0.14 ng/mL) make the detection of PGP-1 more accurate. HepG2 cells, RAW264.7 cells and mice are used to detect the relationship between the expression of PGP-1 and inflammation, and the expression of PGP-1 in different organs and tumors mice was explored for the first time. In vivo experiments have shown that in addition to the up-regulation of PGP-1 in inflammation, the expression of PGP-1 in tumor mice is more.

引用

页数：8

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