Purpose: The ann of the Study was to assess the vision-specific quality-of-life (VS-QoL) of Greek glaucoma patients, and the impact of potential influencing factors. Methods: This was 2-center, cross-sectional Study. One hundred twenty-one patients were recruited from the outpatient glaucoma service and 100 successfully responded to the self-administered, Greek version of the National Eye Institute Visual Function Questionnaire 25. The results were quantified in terms of scores (0-100) and correlations with possible modifiers were investigated. The effects of sex. income, education, and comorbidities oil VS-QoL scores were examined by analysis of variance. Results: Our sample consisted of 49 men and 51 women with a mean age of 64.1 years. ranging from IS to 89 years. The QoL score (mean +/- SD) was 81.7 +/- 14.7, the mean general health subscale score vas 57.3 +/- 21.0 and the mean general vision subscale score was 72.7 +/- 16.3. Men generally presented higher VS-QoL scores (P = 0.042). Age had a negative impact oil the "General health" (rho = -0.325, P = 0.001) and "General vision" (rho = -0.265, P = 0.008) subscales. Higher educational background contributed to higher scores in General health. "General vision," and "Central vision" while urban residence correlated with "Distant activities" and "Social functioning". Cup-to-disc ratio. visual acuity and visual field indices like the Advanced Glaucoma Intervention Study, the Hodapp-Anderson-Parrish, and the Collaborative Initial Glaucoma Treatment Study Scores, and pattern standard deviation and mean deviation all correlated with National Eye Institute Visual Function Questionnaire scores (r values ranging from - 0.240 to 0.757). Conclusions: In this first Study in a Greek native population, both the structural measure of cup-to-disc ratio and a Multitude of functional scores Correlated with VS-QoL scores in glaucoma patients.