Improvement in the expression efficiency of mammalian cytochrome P450 and NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae

被引：3

作者：

Ohgiya, S ^{[1
]}

Kii, T ^{[1
]}

Goda, T ^{[1
]}

Hoshino, T ^{[1
]}

Hamabuchi, K ^{[1
]}

Yokota, H ^{[1
]}

Yuasa, A ^{[1
]}

Ishizaki, K ^{[1
]}

机构：

[1] RAKUNO GAKUEN UNIV,SCH VET MED,DEPT VET BIOCHEM,EBETSU,HOKKAIDO 069,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 05期

关键词：

D O I：

10.1023/A:1018340026148

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mammalian cytochrome P450 (P450) cDNAs were modified by partial or complete removal of their untranslated regions (UTRs). Expression efficiency of P450s in Saccharomyces cerevisiae was increased by the complete removal of the UTRs from the P450 cDNAs prior to insertion into an expression vector. A similar modification was effective in improving the expression of mammalian NADPH-P450 oxidoreductases in S. cerevisiae.

引用

页码：437 / 441

页数：5

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