Deficiency for Lcn8 causes epididymal sperm maturation defects in mice

被引：22
作者：
Wen, Zongzhuang [1 ,2 ]
Liu, Dongyue [1 ,2 ]
Zhu, Haixia [1 ,2 ]
Sun, Xiaoyang [1 ,2 ]
Xiao, Yu [1 ,2 ]
Lin, Zhuchun [3 ]
Zhang, Aizhen [1 ,2 ]
Ye, Chao [1 ,2 ]
Gao, Jiangang [1 ,2 ]
机构：
[1] Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China
[2] Shandong Univ, Key Lab, Minist Educ Expt Teratol, Jinan 250100, Shandong, Peoples R China
[3] Jinan First Peoples Hosp, Jinan 250011, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 548卷
关键词：
Lcn8; Lcn9; Sperm maturation; Epididymis; Mouse model; CRISPR/Cas9;
D O I：
10.1016/j.bbrc.2021.02.052
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lipocalin family members, LCN8 and LCN9, are specifically expressed in the initial segment of mouse caput epididymis. However, the biological functions of the molecules in vivo are yet to be clarified. In this study, CRISPR/Cas9 technology was used to generate Lcn8 and Lcn9 knockout mice, respectively. Lcn8(-/-) and Lcn9(-/-) male mice showed normal spermatogenesis and fertility. In the cauda epididymis of Lcn8(-/-) male mice, morphologically abnormal sperm was increased significantly, the proportion of progressive motility sperm was decreased, the proportion of immobilized sperm was elevated, and the sperm spontaneous acrosome reaction (AR) frequency was increased. Conversely, the knockout of Lcn9 did not have any effect on the ratio of morphologically abnormal sperm, sperm motility, and sperm spontaneous AR frequencies. These results demonstrated the role of LCN8 in maintaining the sperm quality in the epididymis, and suggested that the deficiency of LCN8 leads to epididymal sperm maturation defects. (C) 2021 Elsevier Inc. All rights reserved.
引用
收藏
页码：7 / 13
页数：7
相关论文
共 40 条
  • [1] A unique view on male infertility around the globe
    Agarwal, Ashok
    Mulgund, Aditi
    Hamada, Alaa
    Chyatte, Michelle Renee
    [J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2015, 13
  • [2] MOLECULAR-CLONING OF HUMAN VONEBNERS GLAND PROTEIN, A MEMBER OF THE LIPOCALIN SUPERFAMILY HIGHLY EXPRESSED IN LINGUAL SALIVARY-GLANDS
    BLAKER, M
    KOCK, K
    AHLERS, C
    BUCK, F
    SCHMALE, H
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1172 (1-2) : 131 - 137
  • [3] Lipocalins and cancer
    Bratt, T
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1482 (1-2): : 318 - 326
  • [4] Lipopolysaccharide-Induced Epididymitis Disrupts Epididymal Beta-Defensin Expression and Inhibits Sperm Motility in Rats
    Cao, Dongmei
    Li, Yidong
    Yang, Rui
    Wang, Yan
    Zhou, Yuchuan
    Diao, Hua
    Zhao, Yue
    Zhang, Yonglian
    Lu, Jian
    [J]. BIOLOGY OF REPRODUCTION, 2010, 83 (06) : 1064 - 1070
  • [5] The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer
    Chakraborty, Subhankar
    Kaur, Sukhwinder
    Guha, Sushovan
    Batra, Surinder K.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2012, 1826 (01): : 129 - 169
  • [6] Update on the human and mouse lipocalin (LCN) gene family, including evidence the mouse Mup cluster is result of an "evolutionary bloom"
    Charkoftaki, Georgia
    Wang, Yewei
    McAndrews, Monica
    Bruford, Elspeth A.
    Thompson, David C.
    Vasiliou, Vasilis
    Nebert, Daniel W.
    [J]. HUMAN GENOMICS, 2019, 13 (1) : 11
  • [7] Chu ST, 2000, MOL REPROD DEV, V57, P26, DOI 10.1002/1098-2795(200009)57:1<26::AID-MRD5>3.0.CO
  • [8] 2-4
  • [9] New insights into epididymal function in relation to sperm maturation
    Dacheux, Jean-Louis
    Dacheux, Francoise
    [J]. REPRODUCTION, 2014, 147 (02) : R27 - R42
  • [10] Human plasma lipocalins and serum albumin: Plasma alternative carriers?
    di Masi, Alessandra
    Trezza, Viviana
    Leboffe, Loris
    Ascenzi, Paolo
    [J]. JOURNAL OF CONTROLLED RELEASE, 2016, 228 : 191 - 205
1234