Jasmonate-induced biosynthesis of steroidal glycoalkaloids depends on COI1 proteins in tomato

被引：42

作者：

Abdelkareem, Ayman ^{[1
,2
]}

Thagun, Chonprakun ^{[1
]}

Nakayasu, Masaru ^{[3
]}

Mizutani, Masaharu ^{[3
]}

Hashimoto, Takashi ^{[1
]}

Shoji, Tsubasa ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Takayama 8916-5, Ikoma, Nara 6300101, Japan

[2] Al Azhar Univ, Fac Sci, Cairo, Egypt

[3] Kobe Univ, Grad Sch Agr Sci, Rokkoudai 1-1, Kobe, Hyogo 6578501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 489卷 / 02期

基金：

日本学术振兴会;

关键词：

alpha-tomatine; COI1; Jasmonate; Steroidal glycoalkaloid; Tomato; NICOTINE BIOSYNTHESIS; ALKALOID BIOSYNTHESIS; GENE-EXPRESSION; PLANT; DEFENSE; TOBACCO;

D O I：

10.1016/j.bbrc.2017.05.132

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In tomato, perception of jasmonates by a receptor complex, which includes the F-box protein CORONATINE INSENSITIVE 1 (COI1), elicits biosynthesis of defensive steroidal glycoalkaloids (SGAs) via a jasmonate-responsive ERF transcription factor, JRE4/GAME9. Although JRE4 is upregulated by jasmonate and induces the expression of many metabolic genes involved in SGA biosynthesis, it is not known whether JRE4 alone is sufficient for increased SGA biosynthesis upon activation of jasmonate signaling. Here, we show that application of methyl jasmonate induces the expression of JRE4 and SGA biosynthesis genes in leaves and hairy roots of wild-type tomato, but not in jasmonic acid insensitive I (jail), a loss-of-function mutant allele of the tomato COI1 gene. Induced overexpression of JRE4 increased the expression of SGA biosynthesis genes in transgenic hairy roots of both wild-type tomato and the jail mutant, suggesting that JRE4 is the primary transcription factor that functions downstream of the jasmonate signaling pathway. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：206 / 210

页数：5

共 23 条

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