MicroRNA Mimics or Inhibitors as Antiviral Therapeutic Approaches Against COVID-19

被引:67
作者
Hum, Christine [1 ]
Loiselle, Julia [1 ]
Ahmed, Nadine [1 ]
Shaw, Tyler A. [1 ]
Toudic, Caroline [1 ]
Pezacki, John Paul [1 ,2 ]
机构
[1] Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON K1N 6N5, Canada
[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON K1H 8M5, Canada
基金
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词
A VIRUS-INFECTION; CELLULAR MICRORNAS; OXIDATIVE STRESS; HOST MICRORNAS; EXPRESSION; CORONAVIRUS; TARGETS; PROTEIN; RNA; APOPTOSIS;
D O I
10.1007/s40265-021-01474-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) responsible for the coronavirus disease 2019 (COVID-19) pandemic, present a significant threat to human health by inflicting a wide variety of health complications and even death. While conventional therapeutics often involve administering small molecules to fight viral infections, small non-coding RNA sequences, known as microRNAs (miRNAs/miR-), may present a novel antiviral strategy. We can take advantage of their ability to modulate host-virus interactions through mediating RNA degradation or translational inhibition. Investigations into miRNA and SARS-CoV-2 interactions can reveal novel therapeutic approaches against this virus. The viral genomes of SARS-CoV-2, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle East respiratory syndrome coronavirus (MERS-CoV) were searched using the Nucleotide Basic Local Alignment Search Tool (BLASTn) for highly similar sequences, to identify potential binding sites for miRNAs hypothesized to play a role in SARS-CoV-2 infection. miRNAs that target angiotensin-converting enzyme 2 (ACE2), the receptor used by SARS-CoV-2 and SARS-CoV for host cell entry, were also predicted. Several relevant miRNAs were identified, and their potential roles in regulating SARS-CoV-2 infections were further assessed. Current treatment options for SARS-CoV-2 are limited and have not generated sufficient evidence on safety and efficacy for treating COVID-19. Therefore, by investigating the interactions between miRNAs and SARS-CoV-2, miRNA-based antiviral therapies, including miRNA mimics and inhibitors, may be developed as an alternative strategy to fight COVID-19.
引用
收藏
页码:517 / 531
页数:15
相关论文
共 102 条
[1]   Predicting effective microRNA target sites in mammalian mRNAs [J].
Agarwal, Vikram ;
Bell, George W. ;
Nam, Jin-Wu ;
Bartel, David P. .
ELIFE, 2015, 4
[2]  
[Anonymous], 1988, NUCLEOTIDE
[3]   The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities [J].
Arisan, Elif Damla ;
Dart, Alwyn ;
Grant, Guy H. ;
Arisan, Serdar ;
Cuhadaroglu, Songul ;
Lange, Sigrun ;
Uysal-Onganer, Pinar .
VIRUSES-BASEL, 2020, 12 (06)
[4]   Respiratory syncytial virus modifies microRNAs regulating host genes that affect virus replication [J].
Bakre, Abhijeet ;
Mitchell, Patricia ;
Coleman, Jonathan K. ;
Jones, Les P. ;
Saavedra, Geraldine ;
Teng, Michael ;
Tompkins, S. Mark ;
Tripp, Ralph A. .
JOURNAL OF GENERAL VIROLOGY, 2012, 93 :2346-2356
[5]   MicroRNA Involvement in Signaling Pathways During Viral Infection [J].
Barbu, Madalina Gabriela ;
Condrat, Carmen Elena ;
Thompson, Dana Claudia ;
Bugnar, Oana Larisa ;
Cretoiu, Dragos ;
Toader, Oana Daniela ;
Suciu, Nicolae ;
Voinea, Silviu Cristian .
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2020, 8
[6]   SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs [J].
Bartoszewski, Rafal ;
Dabrowski, Michal ;
Jakiela, Bogdan ;
Matalon, Sadis ;
Harrod, Kevin S. ;
Sanak, Marek ;
Collawn, James F. .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2020, 319 (03) :L444-L455
[7]  
Bonneau E, 2019, EJIFCC, V30, P114
[8]   Pairing beyond the Seed Supports MicroRNA Targeting Specificity [J].
Broughton, James P. ;
Lovci, Michael T. ;
Huang, Jessica L. ;
Yeo, Gene W. ;
Pasquinelli, Amy E. .
MOLECULAR CELL, 2016, 64 (02) :320-333
[9]   Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity [J].
Cantuti-Castelvetri, Ludovico ;
Ojha, Ravi ;
Pedro, Liliana D. ;
Djannatian, Minou ;
Franz, Jonas ;
Kuivanen, Suvi ;
van der Meer, Franziska ;
Kallio, Katri ;
Kaya, Tugberk ;
Anastasina, Maria ;
Smura, Teemu ;
Levanov, Lev ;
Szirovicza, Leonora ;
Tobi, Allan ;
Kallio-Kokko, Hannimari ;
Osterlund, Pamela ;
Joensuu, Merja ;
Meunier, Frederic A. ;
Butcher, Sarah J. ;
Winkler, Martin Sebastian ;
Mollenhauer, Brit ;
Helenius, Ari ;
Gokce, Ozgun ;
Teesalu, Tambet ;
Hepojoki, Jussi ;
Vapalahti, Olli ;
Stadelmann, Christine ;
Balistreri, Giuseppe ;
Simons, Mikael .
SCIENCE, 2020, 370 (6518) :856-+
[10]   Respiratory Syncytial Virus Infection Changes Cargo Composition of Exosome Released from Airway Epithelial Cells [J].
Chahar, Harendra Singh ;
Corsello, Tiziana ;
Kudlicki, Andrzej S. ;
Komaravelli, Narayana ;
Casola, Antonella .
SCIENTIFIC REPORTS, 2018, 8