Angiogenesis function of salidroside in myocardium of rats with myocardial ischemia

This study is to determine the effect of salidroside (SAL) on myocardium of rats with myocardial infarction (MI) and to analyze its possible mechanism. Left coronary artery of Sprague-Dawley rats was ligated to establish MI model. The rats were randomly divided into model group, SAL groups (10, 20, and 40 mg/(kg/d) groups), and the sham-operated group, with 8 rats per group. Hemodynamic changes in rats were determined, and the segmental heart samples were used for H&E or Masson staining. Expression of vascular endothelial growth factors (VEGF) and cluster of differentiation 34 (CD34) were analyzed by semi-quantitative PCR and Western blot. Compared with the sham-operated group, myocardial tissue was damaged and the collagen volume fraction was significantly increased (P<0.01). Compared with the model group, the number of angiogenesis in all the SAL treatment groups was significantly increased while the collagen volume fraction was significantly decreased (P<0.01). In addition, mRNA and protein levels of VEGF and CD34 in the cytoplasm of SAL treated myocardial tissues were significantly increased compared with the model group (P<0.01). SAL can obviously promote angiogenesis in myocardial tissue of rats after MI.