Development and characterization of a PLGA-HA composite material to fabricate 3D-printed scaffolds for bone tissue engineering

被引:93
作者
Babilotte, Joanna [1 ]
Martin, Benoit [1 ]
Guduric, Vera [1 ]
Bareille, Reine [1 ]
Agniel, Remy [2 ]
Roques, Samantha [3 ]
Heroguez, Valerie [4 ]
Dussauze, Marc [5 ]
Gaudon, Manuel [6 ]
Le Nihouannen, Damien [1 ]
Catros, Sylvain [7 ]
机构
[1] Univ Bordeaux, INSERM, BioTis U1026, 146 Rue Leo Saignat, F-33076 Bordeaux, France
[2] CY Cergy Paris Univ, Maison Int Rech, Lab ERRMECe, F-95031 Neuville Oise, France
[3] Univ Bordeaux, CIC1401, CHU Bordeaux, INSERM, F-33076 Bordeaux, France
[4] CNRS, Bordeaux INP, Lab Chim Polymeres Organ, UMR5629, F-33607 Pessac, France
[5] Univ Bordeaux, Inst Sci Mol, UMR CNRS 5255, F-33405 Talence, France
[6] Univ Bordeaux, CNRS, Bordeaux INP, ICMCB,UMR5026, F-33608 Pessac, France
[7] Univ Bordeaux, Dent & Oral Hlth Dept, CHU Bordeaux, INSERM,BioTis U1026, F-33076 Bordeaux, France
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 118卷
关键词
Fused deposition modeling; Bone tissue engineering; Composite; Polymer; Hydroxyapatite; HYDROXYAPATITE; DEGRADATION; ACID;
D O I
10.1016/j.msec.2020.111334
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Additive manufacturing is a rising field in bone tissue engineering. Additive fabrication offers reproducibility, high precision and rapid manufacture of custom patient-specific scaffolds. The development of appropriate composite materials for biomedical applications is critical to reach clinical application of these novel biomaterials. In this work, medical grade poly(lactic-co-glycolic) acid (PLGA) was mixed with hydroxyapatite nanoparticles (nHA) to fabricate 3D porous scaffolds by Fused Deposition Modeling. We have first confirmed that the composite material could be printed in a reproductive manner. Physical characterization demonstrated a low degradation of the material during manufacturing steps and an expected loading and homogeneous distribution of nHA. In vitro biodegradation of the scaffolds showed modifications of morphological and physicochemical properties over time. The composite scaffolds were biocompatible and high cell viability was observed in vitro, as well as a maintain of cell proliferation. As expected, the addition of nHA displayed a positive impact on osteodifferentiation in vitro. Furthermore, a limited inflammatory reaction was observed after subcutaneous implantation of the materials in the rat. Overall, this study suggests that this composite material is suitable for bone tissue engineering applications.
引用
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页数:13
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