Analysis of morphology and absorption of calcium and magnesium for calcium phosphate Ca3(PO4)2 in rat's spine

The decline in bone mass is a sign of metabolic diseases called osteoporosis. Osteoporosis can be treated by adding the calcium level in the form of nano sized Ca-3(PO4)(2). One type of calcium which can build and strengthen the bones is Ca-3(PO4)(2). This study aimed to determine the effectively of nano sized Ca-3(PO4)(2) addition which can be absorbed by rat bones and to identify the crystalline structure that was formed in rat bones with a specific purified diet. Nano sized Ca-3(PO4)(2) was varied in a composition 0.5X, 1.0X and 1.5X compared to the normal needs in the purified diet. The rats were harvested in every two weeks, then their spines were taken to be investigated. The rat's spines were examined by using X-Ray Diffraction (XRD) and Atomic Absorption Spectroscopy (AAS). The effective amount of calcium for the rats was at the value of 0.5X. The amount of Ca as a bone mineral increased as the increasing amount of nano sized Ca-3(PO4)(2), while the amount of Mg tended to fluctuate. The crystalline structure of hexagonal type was found in the rat bones with. Lattice parameters of a = 9.50 A and c = 6.83 A.