Characteristics of Helicobacter pylori natural transformation

被引：55

作者：

Israel, DA

Lou, AS

Blaser, MJ

机构：

[1] Vanderbilt Univ, Sch Med, Dept Med, Div Infect Dis, Nashville, TN 37232 USA

[2] VA Med Ctr, Nashville, TN 37232 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 186卷 / 02期

关键词：

natural transformation; genetics; Helicobacter pylori;

D O I：

10.1111/j.1574-6968.2000.tb09117.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

For Helicobacter pylori, which exhibits substantial genetic diversity, many strains are naturally competent for transformation by exogenous DNA. To better understand the mechanism of natural transformation and its role in the generation of diversity, we sought to systematically identify factors important for natural transformation in H. pylori. We now show that the highest frequency of H. pylori transformation occurs when DNA is introduced prior to exponential phase growth, and that it is a saturable phenomenon. That transformation can be inhibited by DNA from Helicobacter (H. pylori and Helicobacter bills) but not Escherichia coli suggests specificity based on DNA source. Finally, the cag island was determined to be unnecessary for high-frequency transformation. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：275 / 280

页数：6

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