Comparison of Laboratory Colonies and Field Populations of Tamarixia radiata, an Ectoparasitoid of the Asian Citrus Psyllid, Using Internal Transcribed Spacer and Cytochrome Oxidase Subunit I DNA Sequences

被引:21
作者
Barr, N. B. [1 ]
Hall, D. G. [2 ]
Weathersbee, A. A., III [2 ]
Nguyen, R. [3 ]
Stansly, P. [4 ]
Qureshi, J. A. [4 ]
Flores, D. [1 ]
机构
[1] USDA APHIS, Ctr Plant Hlth Sci & Technol, Mission Lab, Edinburg, TX 78541 USA
[2] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA
[3] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA
[4] Univ Florida IFAS, SW Florida Res & Educ Ctr, Immokalee, FL 34142 USA
关键词
biocontrol; citrus greening; pathway analysis; DNA barcode; RIBOSOMAL DNA; HEMIPTERA; IDENTIFICATION; PARASITOIDS; HYMENOPTERA; EULOPHIDAE; HOMOPTERA; DIPTERA; EVOLUTION; ABUNDANCE;
D O I
10.1603/029.102.0639
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The genetic diversity of Tamarixia radiata Waterston (Hymenoptera: Eulophidae) laboratory colonies derived from collections in China, northern Vietnam, Pakistan, and a mixed colony from Taiwan and southern Vietnam was evaluated using the internal transcribed spacer (ITS) region 1, ITS-2, and the 5' end of the cytochrome oxidase subunit I gene. The strains share the same ITS sequence, consistent with the morphological hypothesis that the collections represent a single species. The COI marker was variable and could distinguish the northern Vietnam and Pakistan colonies from each other and from the other colonies. Comparison of COI sequences from field-collected populations of Puerto Rico, Guadeloupe, and Texas indicates that Florida is not a likely source of the introduction into Puerto Rico but is a likely source of the introduction into Texas.
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页码:2325 / 2332
页数:8
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