Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

被引:34
作者
Vastano, Marco [1 ]
Corrado, Iolanda [1 ]
Sannia, Giovanni [1 ]
Solaiman, Daniel K. Y. [2 ]
Pezzella, Cinzia [3 ]
机构
[1] Univ Federico II, Dipartimento Sci Chim, VIa Cinthia, I-48126 Naples, Italy
[2] ARS, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA
[3] Univ Federico II, Dipartimento Agr, Via Univ, Portici, Na, Italy
关键词
ESCHERICHIA-COLI; COOKING OIL; ACID; POLY(HYDROXYALKANOATES); TRANSESTERIFICATION; BIOSYNTHESIS; GENE;
D O I
10.1038/s41598-019-50278-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A sustainable bioprocess was developed for the valorization of a no/low value substrate, i.e. waste frying oils (WFOs) with high content of free fatty acids (FFAs), otherwise unsuitable for biodiesel production. The bioprocess was verified using both recombinant (Escherichia coli) and native (Pseudomonas resinovorans) polyhydroxyalkanoates (PHAs) producing cell factories. Microbial fermentation ofWFOs provided a 2-fold advantage: i)the reduction of FFAs content resulting into an upgrading of the "exhausted waste oils" and the production of a bio-based microbial polymer. Proper strain designing and process optimization allowed to achieve up to 1.5 g L-1 of medium chain length, mcl-PHAs, together with an efficient conversion (80% yield) of the treated WFO into biodiesel.
引用
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页数:8
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