Liquid-Liquid Extraction of GSH Using DEHAP/Octanol Reverse Micelles

被引：10
|
作者：
Zhou, Xiaohua [1 ]
Jin, Ting [1 ]
Dong, Lichun [1 ]
Zheng, Shensheng [1 ]
Xiao, Ji [2 ,3 ,4 ]
机构：
[1] Chongqing Univ, Sch Chem & Chem Engn, Chongqing 400030, Peoples R China
[2] SINOPEC, Beijing Yanshan Petrochem Co Ltd, Res Inst, Beijing, Peoples R China
[3] Dev & Reform Commiss Wansheng Dist, Chongqing, Peoples R China
[4] Management Comm Wansheng Ind Pk, Chongqing, Peoples R China
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2009年 / 44卷 / 15期
关键词：
DEHAP reverse micelles; GSH; reverse micellar extraction; AMINO-ACIDS; PROTEIN EXTRACTION; GLUTATHIONE; SEPARATION; SYSTEM; FERMENTATION; STRESS;
D O I：
10.1080/01496390903182321
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this study, di-(2-ethylhexyl) ammonium phosphate (DEHAP)/octanol reverse micellar extraction was investigated and demonstrated to be an effective method to separate GSH from yeast fermentation broth. The effect of several important factors i.e., pH, DEHAP concentration, and type concentration of cations on both extraction and strip-extraction was investigated. The optimum operating conditions for both extraction and strip-extraction were obtained by two groups of orthogonal experiments. At the optimum operating conditions, the yield of GSH can reach 68%. In the lyophilized product, the purity of GSH was higher than 75%, all the proteins can be separated, and only a small portion of amino acids were left.
引用
收藏
页码：3632 / 3649
页数：18
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