Purification and characterization of β-1, 3-glucanase from the secretion of Simira glaziovii Colleters (Rubiaceae)

被引：9
作者：
Vieira, Felipe Almeida
da Cunha, Maura
Klein, Denise Espellet
Carvalho, Andre de Oliveira
Gomes, Valdirene Moreira
机构：
[1] Univ Estadual Norte Fluminense, Lab Fisiol & Bioquin Microorganismos, BR-28015620 Campos Dos Goytacazes, RJ, Brazil
[2] Univ Estadual Norte Fluminense, Lab Biol Celulat & Tecidual, Ctr Biol & Biotecnol, BR-28015620 Campos Dos Goytacazes, RJ, Brazil
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2006年 / 49卷 / 06期
关键词：
beta-1,3-glucanase; PR protein; chitinases; secretory structure;
D O I：
10.1590/S1516-89132006000700004
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study, beta-1,3-glucanase was isolated, from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2.
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收藏
页码：881 / 888
页数：8
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