Identification and Molecular Detection of ChuA gene of Pathogenic Escherichia coli

During the period of October, 25th 2019 and January, 15th 2020, twenty sampleswere collected from different clinical specimens (stool and urine) from patients attended to the (Rapareen Padiatric and Hawler Teaching hospitals) in Erbil city. The sample was bringing to the laboratory and re-identified by performing morphological, cultural, biochemical tests and confirmed by API 20E systems. The isolates appeared to be varied in their resistance to eleven antibiotics; Imipenim (IMP) was the most effective antibiotic against all isolates of E. coliand all isolates were resistant 100%, to Ticarcillin (TIC), Cephalothin(CEP) and Amoxycillin+ Clavulonic acid (AMC). The resistance rate of the isolates for Amikacin (AK) were 10 %, 50% for Nalidixic acid (NA) and Nitrofurantoin (NIT), 60% for Ceftriaxone (CTR), 65% for Cefotaxime (CTX), 85% for Piperacilin(PI), 95% for Ampicillin(AMP), respectively. AllE. coliisolates were screened for the presence of (ChuA) gene on genomic DNA using polymerase chain reaction (PCR) assay. Results showed that all isolates were positive for the existence of ChuA virulence gene.